Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/YoeB-RelB
Location 3042920..3043449 Replicon chromosome
Accession NZ_CP017755
Organism Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)


Gene name relE Uniprot ID A0A1D9H4T3
Locus tag BKK80_RS32920 Protein ID WP_071018114.1
Coordinates 3042920..3043189 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A1D9H5D1
Locus tag BKK80_RS32925 Protein ID WP_071018112.1
Coordinates 3043186..3043449 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK80_RS32900 3039384..3041054 + 1671 WP_157903438.1 amidohydrolase -
BKK80_RS32905 3041228..3042142 + 915 WP_071072871.1 phosphorylase -
BKK80_RS32910 3042234..3042541 + 308 Protein_2632 hypothetical protein -
BKK80_RS32915 3042585..3042851 - 267 WP_071018116.1 hypothetical protein -
BKK80_RS32920 3042920..3043189 - 270 WP_071018114.1 Txe/YoeB family addiction module toxin Toxin
BKK80_RS32925 3043186..3043449 - 264 WP_071018112.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
BKK80_RS32930 3043525..3044244 - 720 WP_071018110.1 amino acid racemase -
BKK80_RS32935 3044533..3045012 + 480 WP_071039019.1 YiaA/YiaB family protein -
BKK80_RS32940 3045086..3046018 - 933 WP_071073507.1 LysR family transcriptional regulator -
BKK80_RS32945 3046072..3047409 + 1338 WP_157903384.1 MFS transporter -
BKK80_RS32950 3047448..3048422 - 975 WP_071039023.1 tripartite tricarboxylate transporter substrate binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10486.81 Da        Isoelectric Point: 8.0151

>T68111 WP_071018114.1 NZ_CP017755:c3043189-3042920 [Cupriavidus malaysiensis]
MTRQLVFMPDAWDDYLYWQGQDRKTLKRINQLIQDTTRNPFDGVGKPEPLTGNLSGYWSRRIDNTNRLVYHANDDELTII
ACRLHYGAK

Download         Length: 270 bp

>T68111 NZ_CP017755:c3043189-3042920 [Cupriavidus malaysiensis]
ATGACGCGCCAGCTGGTCTTCATGCCCGACGCGTGGGATGACTATCTCTACTGGCAAGGACAGGACAGAAAGACGCTCAA
GCGAATCAATCAGCTCATCCAGGACACCACGCGTAACCCTTTCGATGGAGTCGGCAAGCCTGAGCCCCTGACGGGGAATC
TGTCCGGCTACTGGTCGCGCCGCATCGACAATACCAACCGTCTCGTTTATCACGCCAACGATGACGAGTTGACCATCATT
GCCTGTCGCCTGCACTACGGCGCCAAGTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9935.13 Da        Isoelectric Point: 4.8134

>AT68111 WP_071018112.1 NZ_CP017755:c3043449-3043186 [Cupriavidus malaysiensis]
MRTVHFSDARNNLKAVIDQAIADHDAILITRRDAPNAVIMSQEQYDSWMETMYLLSSPANAERLLRSIEQHRRGEGVERE
LIDPDAA

Download         Length: 264 bp

>AT68111 NZ_CP017755:c3043449-3043186 [Cupriavidus malaysiensis]
ATGCGCACCGTTCATTTCTCCGATGCCCGCAACAACCTGAAGGCCGTGATCGACCAGGCCATCGCCGATCACGATGCCAT
CCTGATCACACGGCGCGATGCGCCCAATGCCGTGATCATGTCGCAGGAACAGTACGACAGCTGGATGGAAACCATGTACC
TGCTCAGTTCGCCGGCCAATGCGGAGCGCTTGCTGCGCTCCATCGAGCAGCATCGCCGGGGCGAGGGGGTGGAGCGTGAG
TTGATCGATCCGGACGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9H4T3

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H5D1

References