Detailed information of TA system TA068109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 680048..680717
• Replicon: chromosome
• Accession: NZ_CP017755
• Organism: Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BKK80_RS22850
• Protein ID: WP_071071425.1
• Coordinates: 680048..680485 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1D9H6W9
• Locus tag: BKK80_RS22855
• Protein ID: WP_071039454.1
• Coordinates: 680472..680717 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK80_RS22840	675529..676806	+	1278	WP_084545711.1	efflux RND transporter periplasmic adaptor subunit	-
BKK80_RS22845	676820..679936	+	3117	WP_071071423.1	efflux RND transporter permease subunit	-
BKK80_RS22850	680048..680485	-	438	WP_071071425.1	PIN domain-containing protein	Toxin
BKK80_RS22855	680472..680717	-	246	WP_071039454.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BKK80_RS22860	680773..681582	-	810	WP_071016813.1	LytTR family DNA-binding domain-containing protein	-
BKK80_RS22865	681590..682687	-	1098	WP_157903407.1	histidine kinase	-
BKK80_RS22870	682794..683756	-	963	WP_071071428.1	sterol desaturase family protein	-
BKK80_RS22875	683848..684360	-	513	WP_071016808.1	hypothetical protein	-
BKK80_RS22880	684678..685601	-	924	WP_071016807.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (18-125)

Antitoxin

• SpoVT-AbrB domain (5-35)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15675.14 Da
• Isoelectric Point: 6.0806

>T68109 WP_071071425.1 NZ_CP017755:c680485-680048 [Cupriavidus malaysiensis]
MGGIESSRAAGRGKAEGFLDSNVLLYLLSADAAKADRAEALLRSRPVISVQVLNEVTHVCVRKLGMRWDEVDEFLTLVKS
LCRIAPLTVDMHDLARRLAARYGLAFYDACIVACASLEGCETLYSEAMHHGLAVEGGPVLANPFP

Nucleotide

• Length: 438 bp

>T68109 NZ_CP017755:c680485-680048 [Cupriavidus malaysiensis]
ATGGGCGGGATTGAGTCCTCCCGCGCAGCGGGTCGCGGCAAGGCCGAAGGATTCCTTGACAGCAATGTCCTGCTCTACCT
GCTGTCGGCCGATGCCGCCAAGGCCGACCGCGCCGAAGCGCTGCTGCGCAGCAGGCCGGTCATCAGCGTCCAGGTGCTGA
ACGAAGTCACCCACGTCTGCGTGCGCAAGCTCGGCATGCGCTGGGACGAAGTCGACGAGTTCCTGACGCTGGTCAAGAGC
CTGTGCCGCATCGCGCCGTTGACCGTGGACATGCACGACCTGGCGCGGCGTCTCGCCGCGCGGTACGGCCTGGCGTTCTA
TGACGCCTGCATCGTGGCTTGTGCGTCGCTCGAGGGCTGCGAGACGCTCTACAGCGAAGCCATGCATCACGGCCTCGCCG
TCGAGGGCGGGCCAGTGCTGGCGAACCCTTTCCCCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9355.53 Da
• Isoelectric Point: 6.3711

>AT68109 WP_071039454.1 NZ_CP017755:c680717-680472 [Cupriavidus malaysiensis]
MQVAKWGNSLAVRLPASVVEALELREGDDIEIVVEQSRTFAIRRKPGTDELLQRLRRFRGRLPADFKFSRDDANERDDGR
D

Nucleotide

• Length: 246 bp

>AT68109 NZ_CP017755:c680717-680472 [Cupriavidus malaysiensis]
ATGCAAGTCGCGAAGTGGGGTAACAGCCTCGCCGTGCGCCTGCCGGCAAGCGTGGTGGAAGCGCTGGAATTGCGCGAGGG
CGACGATATCGAGATCGTGGTCGAGCAGTCCCGCACCTTCGCCATCCGCAGGAAGCCGGGAACGGATGAACTGCTGCAGC
GCCTGCGCCGATTCCGTGGCCGGCTCCCGGCCGACTTCAAGTTCAGCCGCGACGATGCCAACGAGCGGGACGATGGGCGG
GATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D9H6W9