Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-MazE
Location 543949..544544 Replicon chromosome
Accession NZ_CP017755
Organism Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)


Gene name chpB Uniprot ID A0A1D9H6T2
Locus tag BKK80_RS22295 Protein ID WP_071039392.1
Coordinates 544194..544544 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A1D9H6K6
Locus tag BKK80_RS22290 Protein ID WP_071039391.1
Coordinates 543949..544200 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK80_RS22280 541808..543046 - 1239 WP_071016974.1 type II toxin-antitoxin system HipA family toxin -
BKK80_RS22285 543043..543405 - 363 WP_071039390.1 helix-turn-helix transcriptional regulator -
BKK80_RS22290 543949..544200 + 252 WP_071039391.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKK80_RS22295 544194..544544 + 351 WP_071039392.1 type II toxin-antitoxin system ChpB family toxin Toxin
BKK80_RS36600 544510..544632 + 123 WP_157903308.1 DUF1484 family protein -
BKK80_RS22300 544706..545119 - 414 WP_157903309.1 cupin domain-containing protein -
BKK80_RS22305 545200..546027 - 828 WP_071071334.1 AraC family transcriptional regulator -
BKK80_RS22310 546130..546546 - 417 WP_071071336.1 NUDIX domain-containing protein -
BKK80_RS36605 546670..547119 - 450 WP_157903310.1 hypothetical protein -
BKK80_RS36610 547179..547997 - 819 WP_157903311.1 hypothetical protein -
BKK80_RS36615 548120..548938 - 819 WP_157903312.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 509204..553041 43837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-116)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12344.15 Da        Isoelectric Point: 6.3482

>T68108 WP_071039392.1 NZ_CP017755:544194-544544 [Cupriavidus malaysiensis]
MVRRAKFDRGDIVRVSLNPTEGREQQGDFRPALVLSPAAFNALGVALVAPITQGGDFARFAGFAVPLSGAGTETQGVALV
NMVRTLDLEARGARKIERAPAEVVEDALARLQTILD

Download         Length: 351 bp

>T68108 NZ_CP017755:544194-544544 [Cupriavidus malaysiensis]
ATGGTGAGGCGCGCGAAGTTCGACCGGGGCGACATTGTGCGGGTGAGCCTGAACCCAACGGAGGGGCGTGAGCAGCAAGG
GGATTTCCGTCCCGCGCTCGTGCTGTCACCGGCAGCATTCAACGCTCTGGGTGTTGCCCTGGTGGCACCGATTACGCAGG
GCGGCGATTTCGCTCGATTCGCCGGATTTGCCGTACCGTTGTCCGGGGCGGGCACGGAGACTCAAGGCGTCGCGCTGGTG
AACATGGTGCGCACTCTGGATCTTGAGGCTCGCGGTGCGAGGAAGATCGAACGGGCACCTGCCGAGGTCGTGGAAGATGC
GCTCGCCCGTCTTCAAACCATCCTGGATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9141.53 Da        Isoelectric Point: 5.8125

>AT68108 WP_071039391.1 NZ_CP017755:543949-544200 [Cupriavidus malaysiensis]
MELSIQKWGNSAAVRLPTTLLEQLKVALGDKLSVEVRPDGVMLKPARHKYSLDELLAQCDSKAPTPEGLDDWSGAKPVGR
EVW

Download         Length: 252 bp

>AT68108 NZ_CP017755:543949-544200 [Cupriavidus malaysiensis]
ATGGAACTGTCGATTCAGAAATGGGGTAACAGCGCGGCCGTGCGCCTGCCCACGACCTTGCTTGAACAACTCAAGGTGGC
GTTGGGCGACAAACTGTCCGTGGAGGTGCGTCCGGATGGTGTCATGCTTAAACCAGCCAGGCACAAGTATTCACTCGATG
AACTGCTTGCGCAGTGCGACTCCAAGGCGCCGACGCCTGAAGGGCTGGATGATTGGAGCGGGGCTAAGCCGGTTGGGCGC
GAAGTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9H6T2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H6K6

References