Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 304021..305755 Replicon chromosome
Accession NZ_CP017755
Organism Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)


Gene name doc Uniprot ID A0A1D9HB80
Locus tag BKK80_RS21250 Protein ID WP_071040378.1
Coordinates 304021..304887 (+) Length 289 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A1D9H639
Locus tag BKK80_RS21255 Protein ID WP_071039249.1
Coordinates 304925..305755 (+) Length 277 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK80_RS21230 301218..301523 + 306 WP_071017289.1 hypothetical protein -
BKK80_RS21235 301574..302629 + 1056 WP_071073170.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
BKK80_RS21240 302622..303392 + 771 WP_071039248.1 SDR family oxidoreductase -
BKK80_RS21245 303411..303950 + 540 WP_071017285.1 cupin domain-containing protein -
BKK80_RS21250 304021..304887 + 867 WP_071040378.1 alpha/beta hydrolase Toxin
BKK80_RS21255 304925..305755 + 831 WP_071039249.1 IclR family transcriptional regulator Antitoxin
BKK80_RS21260 305772..306575 + 804 WP_071071091.1 SDR family oxidoreductase -
BKK80_RS21265 306568..308295 + 1728 WP_071071094.1 thiamine pyrophosphate-binding protein -
BKK80_RS21270 308312..309112 + 801 WP_071071096.1 aspartate dehydrogenase -
BKK80_RS21275 309156..310631 + 1476 WP_071039253.1 aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(34-270)

Antitoxin

Transcription regulator IclR, C-terminal

(138-258)


Sequences

Toxin        


Download         Length: 289 a.a.        Molecular weight: 29552.00 Da        Isoelectric Point: 8.4856

>T68106 WP_071040378.1 NZ_CP017755:304021-304887 [Cupriavidus malaysiensis]
MLAELDAGFAERTAVLADGARVAYRSGGRQGPLVVLLHGISSGAASWLPCASLLAAQARVLAWDAPGYGASAALAQPAPR
ASDYAARLAGLLEALALRPDVIVGHSLGALMAAAFVAQARPALLPSRLLLLSPAQGYGAPGQQEQAAQVSAQRLQALAQL
GVEGLAERGPQRLLSAAADAGARDWVRWNMRRLNVDGYRQAVAMLCGDAIEPYLQRRAAGVDARVACGGADVVTTPAASE
ALAQRLGLPFGLVPDAGHVCYIEQPQALARMLRGLLPAAAAPGAAVSH

Download         Length: 867 bp

>T68106 NZ_CP017755:304021-304887 [Cupriavidus malaysiensis]
CTGCTCGCCGAACTGGACGCGGGCTTCGCCGAGCGCACGGCCGTGCTGGCCGACGGCGCCCGCGTGGCCTACCGCAGCGG
CGGCAGGCAGGGCCCGCTGGTGGTGCTGCTGCACGGCATCAGCTCCGGCGCGGCCTCCTGGCTGCCGTGCGCCAGCCTGC
TGGCGGCGCAGGCGCGCGTGCTGGCATGGGACGCGCCCGGCTACGGCGCTTCCGCCGCGCTGGCGCAACCGGCGCCGCGC
GCCTCGGACTATGCGGCGCGGCTGGCGGGTTTGCTGGAGGCGCTGGCGCTGCGTCCCGACGTCATCGTCGGCCACTCGCT
GGGCGCGCTGATGGCCGCGGCCTTCGTCGCGCAGGCGCGTCCCGCGCTGCTGCCGTCGCGGCTGCTGCTGCTCAGCCCGG
CGCAGGGCTACGGCGCGCCCGGCCAGCAGGAACAAGCGGCACAGGTGTCGGCGCAGCGGCTGCAGGCGCTGGCGCAGCTC
GGCGTGGAAGGCCTGGCCGAGCGCGGTCCGCAGCGCCTGCTGAGCGCGGCCGCCGATGCCGGCGCGCGCGACTGGGTGCG
CTGGAACATGCGGCGCCTGAACGTGGACGGTTACCGCCAGGCGGTGGCCATGCTGTGCGGCGATGCCATCGAGCCCTACC
TGCAGCGGCGAGCCGCCGGGGTGGACGCGCGGGTGGCCTGCGGCGGCGCGGACGTGGTCACCACGCCGGCGGCGAGCGAG
GCGCTGGCACAGCGTCTGGGCCTGCCTTTCGGCCTGGTGCCCGATGCCGGTCATGTGTGTTATATCGAGCAGCCACAGGC
ACTGGCGCGCATGCTGCGCGGCCTGCTGCCCGCCGCGGCCGCTCCCGGCGCCGCCGTGTCACACTGA

Antitoxin


Download         Length: 277 a.a.        Molecular weight: 30982.59 Da        Isoelectric Point: 6.0599

>AT68106 WP_071039249.1 NZ_CP017755:304925-305755 [Cupriavidus malaysiensis]
MSKATLPEDLQEKYIVPGLERGLRLLCEFSRKDSTLSAAELARRLQVPRSTVFRLLTTLEMMGFVERTDGGREFRLGMAV
LRLGFEYLASLELTELGRPLLDRLRDDIHYPCNLVVRDGRSIVYVAKSVAPTPFASSVNVGTRLPAHATVLGRVLLEDLS
LAELRELYPESQLEVFSESTPRTVEQLFEMVQHDRQRGYVLQEGFFEASISTIAAPVRDRSGKVAAAMGATIPASRIDPE
QLGPMVEQVRHAADELSRLLDYRPAAEHKVVNLYRE

Download         Length: 831 bp

>AT68106 NZ_CP017755:304925-305755 [Cupriavidus malaysiensis]
ATGAGCAAAGCCACCCTGCCCGAAGACCTCCAGGAAAAGTACATCGTGCCGGGCCTGGAGCGCGGACTGCGGCTGCTGTG
CGAATTCAGCCGCAAGGACAGCACGCTGTCGGCGGCCGAACTGGCGCGCCGGCTGCAGGTGCCCCGCTCCACGGTGTTCC
GTTTGCTGACCACGCTGGAGATGATGGGTTTCGTCGAGCGCACCGACGGCGGGCGCGAGTTCCGCCTCGGCATGGCCGTG
CTGCGCCTGGGCTTCGAGTACCTGGCCTCGCTGGAACTGACCGAGCTGGGCCGGCCGCTGCTCGACCGCCTGCGCGACGA
CATCCACTACCCGTGCAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTCGCCAAGTCGGTGGCGCCGACGCCGT
TCGCCAGCTCCGTCAACGTGGGCACGCGCCTGCCGGCGCACGCCACCGTGCTCGGCCGCGTGCTGCTGGAAGACCTGTCG
CTGGCCGAACTGCGCGAGCTCTATCCGGAGTCGCAGCTCGAGGTGTTCTCGGAAAGCACCCCGCGCACCGTCGAACAGCT
GTTCGAGATGGTGCAGCACGACCGCCAGCGCGGCTACGTGCTGCAGGAAGGCTTCTTCGAAGCGAGCATCTCCACCATCG
CGGCGCCGGTGCGCGATCGCAGCGGCAAGGTGGCGGCGGCGATGGGCGCGACGATTCCGGCCTCGCGCATCGACCCGGAG
CAGCTCGGCCCGATGGTGGAACAGGTCCGTCATGCGGCGGACGAGCTCTCGCGCCTGCTGGACTACCGTCCGGCGGCGGA
GCACAAGGTAGTCAACTTATACCGAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9HB80

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H639

References