Detailed information of TA system TA068104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 381605..383217
• Replicon: chromosome
• Accession: NZ_CP017754
• Organism: Cupriavidus malaysiensis strain USMAA1020 isolate pure

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BKK80_RS01570
• Protein ID: WP_071068490.1
• Coordinates: 381877..383217 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1D9H2X1
• Locus tag: BKK80_RS01565
• Protein ID: WP_071015926.1
• Coordinates: 381605..381871 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK80_RS01540	377729..378472	+	744	WP_071068488.1	3-oxoacid CoA-transferase subunit A	-
BKK80_RS01545	378478..379140	+	663	WP_071010570.1	3-oxoacid CoA-transferase subunit B	-
BKK80_RS01550	379301..380587	+	1287	WP_071068489.1	MFS transporter	-
BKK80_RS01555	380681..381019	+	339	WP_071010572.1	4-methylmuconolactone methylisomerase	-
BKK80_RS01560	381140..381415	+	276	WP_071010573.1	muconolactone Delta-isomerase	-
BKK80_RS01565	381605..381871	+	267	WP_071015926.1	helix-turn-helix domain-containing protein	Antitoxin
BKK80_RS01570	381877..383217	+	1341	WP_071068490.1	type II toxin-antitoxin system HipA family toxin	Toxin
BKK80_RS01575	383539..383775	-	237	WP_071068491.1	hypothetical protein	-
BKK80_RS01580	383934..384809	-	876	WP_157903141.1	anti-sigma factor	-
BKK80_RS01585	384799..385401	-	603	WP_071037678.1	RNA polymerase sigma factor	-
BKK80_RS01590	385522..386043	-	522	WP_084545449.1	dethiobiotin synthetase	-
BKK80_RS01595	386040..386648	-	609	WP_205683715.1	lecithin retinol acyltransferase family protein	-
BKK80_RS01600	386771..387700	-	930	WP_071068492.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (157-386)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-67)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49089.28 Da
• Isoelectric Point: 8.7017

>T68104 WP_071068490.1 NZ_CP017754:381877-383217 [Cupriavidus malaysiensis]
MGRRSRTRSLGLWMNGVYVGNWQFQPQHGDVLQYAEAWLSAPEGRPLSLSLPFTPGNAAHRGDAVRAYFENLLPDSREIR
DRIARRFRTETTDAFDLLAQVGRDCAGALQLLPDGMAPTGVATIDGEPLNEAQMARALRGAVMQQPPAGADDGEFRISIA
GAQEKTAFLWADGQWLRPRGSTPTSHIFKLPMGLVGNMQLDLGESVENEWLCAQILAAYGMPVAACRPLMFENQKVLCVE
RFDRMWSKGSKPWLIRLPQEDMCQATATPPYRKYEADGGPGIDRIMRLLDGSMHREADRLRFFQAQVLFWMLCAPDGHAK
NFSLALRPGGAYQLTPLYDVMSAYPLLGEGPGKLSPHKVRLAMAVRSSNAHWRVRDIRRRHWLELGRRHAIVTPEGGDAE
SVVNGLAASTPAVVTRVRSLLPAGFPSALADTILGGLQAAADKLAA

Nucleotide

• Length: 1341 bp

>T68104 NZ_CP017754:381877-383217 [Cupriavidus malaysiensis]
ATGGGGCGCCGCTCTCGCACGCGCAGCCTCGGACTCTGGATGAACGGCGTCTATGTCGGCAACTGGCAGTTCCAGCCGCA
ACACGGGGACGTACTGCAATATGCGGAGGCCTGGCTTTCGGCGCCCGAAGGGCGTCCGCTGTCCTTGTCGCTGCCGTTCA
CGCCGGGCAACGCCGCGCATCGGGGTGACGCCGTGCGCGCCTACTTCGAAAACCTGCTGCCCGACAGTCGGGAGATACGC
GATCGCATTGCACGGCGTTTCCGCACGGAAACGACCGATGCCTTCGATCTGTTGGCACAAGTCGGGCGCGACTGTGCGGG
CGCACTGCAATTGCTGCCGGACGGCATGGCGCCGACAGGCGTCGCCACGATCGATGGGGAACCACTGAACGAAGCGCAGA
TGGCGAGGGCATTGCGCGGAGCGGTCATGCAGCAGCCGCCAGCCGGGGCCGATGATGGTGAGTTCCGCATATCGATCGCC
GGCGCCCAGGAGAAGACTGCTTTCCTGTGGGCGGACGGTCAATGGTTGCGCCCCAGAGGCTCCACGCCCACCAGTCATAT
CTTCAAGCTCCCCATGGGACTGGTCGGCAATATGCAACTGGACCTGGGCGAATCGGTCGAGAATGAATGGCTGTGCGCAC
AGATCCTCGCGGCCTATGGCATGCCCGTGGCCGCTTGCCGGCCGCTCATGTTCGAGAACCAGAAGGTGCTCTGTGTCGAA
CGCTTCGATCGCATGTGGTCGAAGGGAAGCAAGCCCTGGCTCATCCGCCTGCCTCAGGAGGACATGTGCCAGGCCACCGC
CACGCCGCCATATCGGAAGTACGAAGCCGATGGTGGCCCGGGGATCGACCGCATCATGCGTCTGCTCGATGGCTCGATGC
ATCGTGAGGCGGATCGCCTGCGCTTTTTCCAGGCACAGGTTCTGTTCTGGATGCTGTGCGCCCCCGACGGACATGCCAAG
AATTTCAGCTTGGCGTTGCGTCCTGGTGGCGCCTACCAGCTGACACCCCTATACGATGTGATGTCGGCCTATCCACTGCT
TGGCGAAGGACCAGGGAAACTCTCGCCGCACAAGGTCCGCCTGGCGATGGCGGTCCGTTCCAGCAACGCACATTGGCGAG
TACGGGACATCCGGCGGCGGCACTGGCTGGAACTGGGGCGCCGCCACGCCATCGTCACGCCGGAGGGCGGCGACGCGGAG
TCCGTGGTCAACGGTCTCGCAGCAAGCACGCCTGCGGTAGTCACCCGCGTGCGCAGCCTGTTGCCAGCGGGGTTCCCTTC
GGCACTGGCCGACACCATCCTTGGTGGATTGCAGGCGGCGGCGGACAAGCTTGCGGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9535.01 Da
• Isoelectric Point: 11.0917

>AT68104 WP_071015926.1 NZ_CP017754:381605-381871 [Cupriavidus malaysiensis]
MREFTVRTPEQLPAMLRGFRKQAGLTQAELAARLGVRQQTLSGLERNAESVSAGRLLTLLSVLGVELVLRQADAAAQAAP
PPTDLPQW

Nucleotide

• Length: 267 bp

>AT68104 NZ_CP017754:381605-381871 [Cupriavidus malaysiensis]
ATGCGCGAATTCACGGTCCGCACGCCCGAGCAACTCCCAGCCATGCTCCGAGGCTTCCGCAAGCAAGCCGGCCTGACCCA
GGCCGAACTGGCTGCCCGTCTGGGCGTACGCCAGCAGACATTGTCGGGGCTGGAGCGCAACGCGGAAAGCGTCAGCGCCG
GGCGCCTGTTGACGCTGCTGTCGGTATTGGGTGTCGAACTCGTGCTTCGGCAAGCGGACGCTGCCGCGCAGGCCGCGCCC
CCGCCAACCGATCTTCCTCAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D9H2X1