Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/YoeB-RelB
Location 1139744..1140273 Replicon chromosome
Accession NZ_CP017752
Organism Cupriavidus sp. USMAHM13 isolate pure

Toxin (Protein)


Gene name relE Uniprot ID A0A1D9H4T3
Locus tag BKK81_RS23395 Protein ID WP_071018114.1
Coordinates 1140004..1140273 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A1D9H5D1
Locus tag BKK81_RS23390 Protein ID WP_071018112.1
Coordinates 1139744..1140007 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK81_RS23365 1134765..1135739 + 975 WP_071018103.1 tripartite tricarboxylate transporter substrate binding protein -
BKK81_RS23370 1135778..1137070 - 1293 WP_071018105.1 MFS transporter -
BKK81_RS23375 1137175..1138107 + 933 WP_071018107.1 LysR family transcriptional regulator -
BKK81_RS23380 1138181..1138660 - 480 WP_071018109.1 YiaA/YiaB family protein -
BKK81_RS23385 1138949..1139668 + 720 WP_071018110.1 amino acid racemase -
BKK81_RS23390 1139744..1140007 + 264 WP_071018112.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
BKK81_RS23395 1140004..1140273 + 270 WP_071018114.1 Txe/YoeB family addiction module toxin Toxin
BKK81_RS23400 1140342..1140608 + 267 WP_071018116.1 hypothetical protein -
BKK81_RS23405 1140652..1140956 - 305 Protein_999 tripartite tricarboxylate transporter substrate binding protein -
BKK81_RS23410 1141053..1141967 - 915 WP_071018120.1 phosphorylase -
BKK81_RS23415 1142141..1143835 - 1695 WP_071018122.1 amidohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10486.81 Da        Isoelectric Point: 8.0151

>T68102 WP_071018114.1 NZ_CP017752:1140004-1140273 [Cupriavidus sp. USMAHM13]
MTRQLVFMPDAWDDYLYWQGQDRKTLKRINQLIQDTTRNPFDGVGKPEPLTGNLSGYWSRRIDNTNRLVYHANDDELTII
ACRLHYGAK

Download         Length: 270 bp

>T68102 NZ_CP017752:1140004-1140273 [Cupriavidus sp. USMAHM13]
ATGACGCGCCAGCTGGTCTTCATGCCCGACGCGTGGGATGACTATCTCTACTGGCAAGGACAGGACAGAAAGACGCTCAA
GCGAATCAATCAGCTCATCCAGGACACCACGCGTAACCCTTTCGATGGAGTCGGCAAGCCTGAGCCCCTGACGGGGAATC
TGTCCGGCTACTGGTCGCGCCGCATCGACAATACCAACCGTCTCGTTTATCACGCCAACGATGACGAGTTGACCATCATT
GCCTGTCGCCTGCACTACGGCGCCAAGTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9935.13 Da        Isoelectric Point: 4.8134

>AT68102 WP_071018112.1 NZ_CP017752:1139744-1140007 [Cupriavidus sp. USMAHM13]
MRTVHFSDARNNLKAVIDQAIADHDAILITRRDAPNAVIMSQEQYDSWMETMYLLSSPANAERLLRSIEQHRRGEGVERE
LIDPDAA

Download         Length: 264 bp

>AT68102 NZ_CP017752:1139744-1140007 [Cupriavidus sp. USMAHM13]
ATGCGCACCGTTCATTTCTCCGATGCCCGCAACAACCTGAAGGCCGTGATCGACCAGGCCATCGCCGATCACGATGCCAT
CCTGATCACACGGCGCGATGCGCCCAATGCCGTGATCATGTCGCAGGAACAGTACGACAGCTGGATGGAAACCATGTACC
TGCTCAGTTCGCCGGCCAATGCGGAGCGCTTGCTGCGCTCCATCGAGCAGCATCGCCGGGGCGAGGGGGTGGAGCGTGAG
TTGATCGATCCGGACGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9H4T3

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H5D1

References