Detailed information of TA system TA068099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 140819..141488
• Replicon: chromosome
• Accession: NZ_CP017752
• Organism: Cupriavidus sp. USMAHM13 isolate pure

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BKK81_RS19095
• Protein ID: WP_071016816.1
• Coordinates: 141051..141488 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BKK81_RS19090
• Protein ID: WP_071016815.1
• Coordinates: 140819..141064 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK81_RS19065	135928..136851	+	924	WP_071016807.1	LysR family transcriptional regulator	-
BKK81_RS19070	137160..137672	+	513	WP_071016808.1	hypothetical protein	-
BKK81_RS19075	137764..138726	+	963	WP_071016810.1	sterol desaturase family protein	-
BKK81_RS19080	138833..139930	+	1098	WP_156811524.1	histidine kinase	-
BKK81_RS19085	139938..140747	+	810	WP_071016813.1	LytTR family DNA-binding domain-containing protein	-
BKK81_RS19090	140819..141064	+	246	WP_071016815.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BKK81_RS19095	141051..141488	+	438	WP_071016816.1	PIN domain-containing protein	Toxin
BKK81_RS19100	141600..144716	-	3117	WP_071016818.1	efflux RND transporter permease subunit	-
BKK81_RS19105	144730..146007	-	1278	WP_083384255.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (18-125)

Antitoxin

• SpoVT-AbrB domain (5-35)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15675.14 Da
• Isoelectric Point: 6.0806

>T68099 WP_071016816.1 NZ_CP017752:141051-141488 [Cupriavidus sp. USMAHM13]
MGGIESSRAAGRGKAEGFLDSNVLLYLLSADAAKADRAEALLRSRPVISVQVLNEVTHVCVRKLGMRWDEVDEFLTLVKS
LCRIAPLTVDMHDLARRLAARYGLAFYDACIVACASLEGCETLYSEAMHHGPAVEGGPVLANPFL

Nucleotide

• Length: 438 bp

>T68099 NZ_CP017752:141051-141488 [Cupriavidus sp. USMAHM13]
ATGGGCGGGATTGAGTCCTCCCGCGCAGCGGGTCGCGGCAAGGCCGAAGGATTCCTTGACAGCAATGTCCTGCTCTACCT
GCTGTCGGCCGATGCCGCCAAGGCCGACCGCGCCGAAGCGCTGCTGCGCAGCAGGCCGGTCATCAGCGTCCAGGTGCTGA
ACGAAGTCACCCACGTCTGCGTGCGCAAGCTCGGCATGCGCTGGGACGAAGTCGACGAGTTCCTAACGCTGGTCAAGAGC
CTGTGCCGCATCGCGCCGTTGACCGTGGACATGCACGACCTGGCGCGGCGTCTCGCCGCGCGGTACGGCCTGGCGTTCTA
TGACGCCTGCATCGTGGCTTGTGCGTCGCTCGAGGGCTGCGAGACGCTCTACAGCGAAGCCATGCATCACGGCCCCGCCG
TCGAGGGCGGGCCGGTGCTGGCGAACCCTTTCCTCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9306.50 Da
• Isoelectric Point: 8.9101

>AT68099 WP_071016815.1 NZ_CP017752:140819-141064 [Cupriavidus sp. USMAHM13]
MQVAKWGNSLAVRLPASVVEALELREGDDIEIVVEHSRTFAIRRKPGTDELLQRLRRFRGRLPADFKFSRDDANERGDGR
D

Nucleotide

• Length: 246 bp

>AT68099 NZ_CP017752:140819-141064 [Cupriavidus sp. USMAHM13]
ATGCAAGTCGCGAAGTGGGGTAACAGCCTCGCCGTGCGCCTGCCGGCAAGCGTGGTGGAAGCGCTGGAATTGCGCGAGGG
CGACGATATCGAGATCGTGGTCGAGCATTCCCGCACCTTCGCCATCCGCAGGAAGCCGGGAACGGATGAACTGCTGCAGC
GCCTGCGCCGATTCCGCGGCCGGCTCCCGGCCGACTTCAAGTTCAGCCGCGACGATGCCAACGAGCGGGGCGATGGGCGG
GATTGA