Detailed information of TA system TA068098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1394320..1394904
• Replicon: chromosome
• Accession: NZ_CP017751
• Organism: Cupriavidus sp. USMAHM13 isolate pure

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BKK81_RS06190
• Protein ID: WP_071011398.1
• Coordinates: 1394722..1394904 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1D9GV91
• Locus tag: BKK81_RS06185
• Protein ID: WP_071011396.1
• Coordinates: 1394320..1394712 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK81_RS06160	1389771..1390064	+	294	WP_071011393.1	hypothetical protein	-
BKK81_RS06165	1390123..1390920	+	798	WP_071016142.1	2-keto-4-pentenoate hydratase	-
BKK81_RS06170	1391136..1392014	-	879	Protein_1239	LysR family transcriptional regulator	-
BKK81_RS06175	1392436..1393632	+	1197	WP_071016144.1	DUF4382 domain-containing protein	-
BKK81_RS35185	1393764..1394201	+	438	WP_156811133.1	hypothetical protein	-
BKK81_RS06185	1394320..1394712	-	393	WP_071011396.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BKK81_RS06190	1394722..1394904	-	183	WP_071011398.1	type II toxin-antitoxin system HicA family toxin	Toxin
BKK81_RS06195	1395407..1396462	+	1056	WP_083383970.1	chitinase	-
BKK81_RS06200	1396986..1397834	-	849	WP_071011400.1	hypothetical protein	-
BKK81_RS06205	1398110..1398427	+	318	WP_071011401.1	multidrug efflux SMR transporter	-
BKK81_RS06210	1398581..1398994	-	414	WP_083384204.1	hypothetical protein	-
BKK81_RS06215	1399106..1399489	-	384	WP_156811135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1394320..1407804	13484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6773.78 Da
• Isoelectric Point: 10.3517

>T68098 WP_071011398.1 NZ_CP017751:c1394904-1394722 [Cupriavidus sp. USMAHM13]
MHSADLIRQLKADGWQLVYTVGSHYQFRYPTTPGKVTVPHPDKDLPIGTVRSIQKQAGLR

Nucleotide

• Length: 183 bp

>T68098 NZ_CP017751:c1394904-1394722 [Cupriavidus sp. USMAHM13]
ATGCATAGCGCAGACCTGATCCGGCAACTCAAGGCGGATGGCTGGCAACTCGTCTACACCGTGGGTTCCCACTATCAGTT
CAGGTACCCGACGACGCCGGGAAAGGTAACGGTCCCGCATCCGGACAAGGATCTCCCGATAGGCACGGTGCGCAGTATCC
AGAAACAGGCTGGCTTGAGGTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14353.26 Da
• Isoelectric Point: 4.9323

>AT68098 WP_071011396.1 NZ_CP017751:c1394712-1394320 [Cupriavidus sp. USMAHM13]
MLYPLYVHVGDARHAHGVSFPDFPGCFAAADDWEDLPAAVQEAAEAHFAGSETPIPRPTPLESLTASPEFEGGVWMLFDI
DLSKLRSKAVRLNISLPERLLQQIDAAARQRRLSRSAFLALAAEHEMERA

Nucleotide

• Length: 393 bp

>AT68098 NZ_CP017751:c1394712-1394320 [Cupriavidus sp. USMAHM13]
ATGCTCTATCCCCTGTATGTCCACGTGGGAGACGCCAGGCACGCGCACGGCGTCTCATTCCCCGACTTCCCGGGCTGCTT
TGCCGCGGCAGACGACTGGGAAGATCTGCCCGCCGCCGTGCAGGAGGCCGCCGAAGCCCATTTCGCCGGCAGCGAGACAC
CCATTCCCCGGCCAACGCCATTGGAATCCCTGACCGCATCGCCCGAGTTCGAAGGCGGCGTCTGGATGCTGTTCGACATC
GATCTGTCCAAGCTGAGATCCAAGGCGGTGCGGCTGAATATCAGCCTTCCGGAACGGCTTCTTCAGCAGATCGACGCGGC
CGCCAGGCAGCGGCGCCTGTCGCGCTCGGCCTTCCTCGCCCTGGCGGCGGAGCACGAGATGGAGCGAGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1D9GV91