Detailed information of TA system TA068097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 499445..501057
• Replicon: chromosome
• Accession: NZ_CP017751
• Organism: Cupriavidus sp. USMAHM13 isolate pure

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BKK81_RS02240
• Protein ID: WP_071010574.1
• Coordinates: 499717..501057 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1D9H2X1
• Locus tag: BKK81_RS02235
• Protein ID: WP_071015926.1
• Coordinates: 499445..499711 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK81_RS02210	495545..496288	+	744	WP_071010569.1	3-oxoacid CoA-transferase subunit A	-
BKK81_RS02215	496294..496956	+	663	WP_071010570.1	3-oxoacid CoA-transferase subunit B	-
BKK81_RS02220	497141..498427	+	1287	WP_071010571.1	MFS transporter	-
BKK81_RS02225	498521..498859	+	339	WP_071010572.1	4-methylmuconolactone methylisomerase	-
BKK81_RS02230	498980..499255	+	276	WP_071010573.1	muconolactone Delta-isomerase	-
BKK81_RS02235	499445..499711	+	267	WP_071015926.1	helix-turn-helix domain-containing protein	Antitoxin
BKK81_RS02240	499717..501057	+	1341	WP_071010574.1	type II toxin-antitoxin system HipA family toxin	Toxin
BKK81_RS02245	501380..501616	-	237	WP_071010575.1	hypothetical protein	-
BKK81_RS02250	501776..502576	-	801	WP_071015928.1	anti-sigma factor	-
BKK81_RS02255	502617..503219	-	603	WP_071010576.1	RNA polymerase sigma factor	-
BKK81_RS02260	503340..503861	-	522	WP_083383881.1	dethiobiotin synthetase	-
BKK81_RS02265	503858..504466	-	609	WP_197523960.1	lecithin retinol acyltransferase family protein	-
BKK81_RS02270	504589..505518	-	930	WP_071010577.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-67)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49189.40 Da
• Isoelectric Point: 8.4791

>T68097 WP_071010574.1 NZ_CP017751:499717-501057 [Cupriavidus sp. USMAHM13]
MGRRSRTRSLGLWMNGVYVGNWQFQPQHGDVLQYAEAWLSAPEGRPLSLSLPFTPGNAAHRGDAVRAYFENLLPDSREIR
DRIARRFRTETTDAFDLLAQVGRDCVGALQLLPDGMAPTGVATIDGEPLNEAQMARALRGAVMQQPPAEADDGEFRISIA
GAQEKTAFLWADGQWLRPRGSTPTSHIFKLPMGLVGNMQLDLGESVENEWLCAQILAAYGMPVAACRPLMFENQKVLCVE
RFDRMWSKGSKPWLIRLPQEDMCQATATPPYRKYEADGGPGIDRIMRLLDGSMHREADRLRFFQAQVLFWMLCAPDGHAK
NFSLALRPGGAYQLTPLYDVMSAYPLLGEGPGKLSPHKVRLAMAVRSSNAHWRVRDIRRRHWLELGRRHAIVTPEGGDAE
SVVNGLAASTPAVVTRVRSLLPAGFPSALADTILGGLQAAADKLAA

Nucleotide

• Length: 1341 bp

>T68097 NZ_CP017751:499717-501057 [Cupriavidus sp. USMAHM13]
ATGGGGCGCCGCTCTCGCACGCGCAGCCTCGGACTCTGGATGAACGGCGTCTATGTCGGCAACTGGCAGTTCCAGCCGCA
ACACGGGGACGTACTGCAATATGCGGAGGCCTGGCTTTCGGCGCCCGAAGGGCGTCCGCTGTCCTTGTCGCTGCCGTTCA
CGCCGGGCAACGCCGCGCATCGGGGTGACGCCGTGCGCGCCTACTTCGAAAACCTGCTGCCCGACAGTCGGGAGATACGC
GATCGCATTGCACGGCGTTTCCGCACGGAAACGACCGATGCCTTCGATCTGTTGGCACAAGTCGGGCGCGACTGTGTGGG
CGCACTGCAATTGCTGCCGGACGGCATGGCGCCGACAGGCGTCGCCACGATCGATGGGGAACCACTGAACGAAGCGCAGA
TGGCGAGGGCATTGCGCGGAGCGGTCATGCAGCAGCCGCCAGCCGAGGCCGATGATGGTGAGTTCCGCATATCGATCGCC
GGCGCCCAGGAGAAGACTGCTTTCCTGTGGGCGGACGGTCAATGGTTGCGCCCCAGAGGCTCCACGCCCACCAGTCATAT
CTTCAAGCTCCCCATGGGACTGGTCGGCAATATGCAACTGGACCTGGGCGAATCGGTCGAGAATGAATGGCTGTGCGCAC
AGATCCTCGCGGCCTATGGCATGCCCGTGGCCGCTTGCCGGCCGCTCATGTTCGAGAACCAGAAGGTGCTCTGTGTCGAA
CGCTTCGATCGCATGTGGTCGAAGGGAAGCAAGCCCTGGCTCATCCGCCTGCCTCAGGAGGACATGTGCCAGGCCACCGC
CACGCCGCCATATCGGAAGTACGAAGCCGATGGTGGCCCGGGGATCGACCGCATCATGCGTCTGCTCGATGGCTCGATGC
ATCGTGAGGCGGATCGCCTGCGCTTTTTCCAGGCACAGGTTCTGTTCTGGATGCTGTGCGCCCCCGACGGACATGCCAAG
AATTTCAGCTTGGCGTTGCGTCCTGGTGGCGCCTACCAGCTGACACCCCTATACGATGTGATGTCGGCCTATCCACTGCT
TGGCGAAGGACCAGGGAAACTCTCGCCGCACAAGGTCCGCCTGGCGATGGCGGTCCGTTCCAGCAACGCACATTGGCGAG
TACGGGACATCCGGCGGCGGCACTGGCTGGAACTGGGGCGCCGCCACGCCATCGTCACGCCGGAGGGCGGCGACGCGGAG
TCCGTGGTCAACGGTCTCGCAGCAAGCACGCCTGCGGTAGTCACCCGCGTGCGCAGCCTGTTGCCAGCGGGGTTCCCTTC
GGCACTGGCCGACACCATCCTTGGTGGATTGCAGGCGGCGGCGGACAAGCTTGCGGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9535.01 Da
• Isoelectric Point: 11.0917

>AT68097 WP_071015926.1 NZ_CP017751:499445-499711 [Cupriavidus sp. USMAHM13]
MREFTVRTPEQLPAMLRGFRKQAGLTQAELAARLGVRQQTLSGLERNAESVSAGRLLTLLSVLGVELVLRQADAAAQAAP
PPTDLPQW

Nucleotide

• Length: 267 bp

>AT68097 NZ_CP017751:499445-499711 [Cupriavidus sp. USMAHM13]
ATGCGCGAATTCACGGTCCGCACGCCCGAGCAACTCCCAGCCATGCTCCGAGGCTTCCGCAAGCAAGCCGGCCTGACCCA
GGCCGAACTGGCTGCCCGTCTGGGCGTACGCCAGCAGACATTGTCGGGGCTGGAGCGCAACGCGGAAAGCGTCAGCGCCG
GGCGCCTGTTGACGCTGCTGTCGGTATTGGGTGTCGAACTCGTGCTTCGGCAAGCGGACGCTGCCGCGCAGGCCGCGCCC
CCGCCAACCGATCTTCCTCAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D9H2X1