Detailed information of TA system TA068096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 523382..524075
• Replicon: plasmid unnamed1
• Accession: NZ_CP017750
• Organism: Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A1D9HD98
• Locus tag: BKK79_RS38515
• Protein ID: WP_071040659.1
• Coordinates: 523698..524075 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A1D9HD92
• Locus tag: BKK79_RS38510
• Protein ID: WP_071040658.1
• Coordinates: 523382..523717 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK79_RS38465	519536..519784	-	249	WP_071040846.1	DUF2442 domain-containing protein	-
BKK79_RS39965	519768..519983	-	216	WP_084085842.1	DUF4160 domain-containing protein	-
BKK79_RS38470	520016..520573	+	558	WP_071040847.1	hypothetical protein	-
BKK79_RS38475	520677..520928	+	252	WP_071040848.1	hypothetical protein	-
BKK79_RS38480	521039..521332	-	294	WP_071040878.1	HigA family addiction module antidote protein	-
BKK79_RS38485	521403..521774	-	372	WP_071040654.1	hypothetical protein	-
BKK79_RS38490	521771..522271	-	501	WP_071040855.1	transposase	-
BKK79_RS38495	522268..522594	-	327	WP_071040655.1	hypothetical protein	-
BKK79_RS38500	522618..523013	-	396	WP_071040656.1	hypothetical protein	-
BKK79_RS38505	523010..523369	-	360	WP_071040657.1	transposase	-
BKK79_RS38510	523382..523717	-	336	WP_071040658.1	helix-turn-helix domain-containing protein	Antitoxin
BKK79_RS38515	523698..524075	-	378	WP_071040659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKK79_RS38520	524267..524860	+	594	WP_071040660.1	recombinase family protein	-
BKK79_RS38525	524857..527886	+	3030	WP_071040661.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..544877	544877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13758.90 Da
• Isoelectric Point: 8.8594

>T68096 WP_071040659.1 NZ_CP017750:c524075-523698 [Cupriavidus sp. USMAA2-4]
MVDKEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEALAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T68096 NZ_CP017750:c524075-523698 [Cupriavidus sp. USMAA2-4]
ATGGTCGATAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCTACAAGGATCTGATGGCGTTGCCGCCTGACGTGCGTCG
GCGTTTCGGTTACGCGCTGTCGCTGGCCCAGATGGGCGACCAGGACGACGCGGCGAAGGTGCTCAAGGGCTTCGGCGGGG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACGTATCGGGCGGTCTACACGGTGAAGTTCGCGGAAGCGGTG
TTTGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCCAAGGTGGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGCATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12154.33 Da
• Isoelectric Point: 10.3419

>AT68096 WP_071040658.1 NZ_CP017750:c523717-523382 [Cupriavidus sp. USMAA2-4]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVIEIRKAMRALGLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT68096 NZ_CP017750:c523717-523382 [Cupriavidus sp. USMAA2-4]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAACGCAGCTCGGGCAACGTCTTTGCCGATCTGGGACTGCCCGATGC
CGAGAAACTGAAGATCAAGACCGGCCTGGTGATCGAGATCAGGAAGGCGATGCGGGCCCTTGGCCTGACACAACAGGCGG
CGGCCAAGCGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCACGGCGATTTCACCAATCTGTCCGAACGCAAG
CTGATGGACTGCCTGAACCGTCTCGGCTACGACATCGAAATCAAGGTGCGGCCGGCGGCCGAGCCAGTCGGGCATCTGAC
GCTCGCCACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1D9HD98

Antitoxin

• AlphaFold DB: A0A1D9HD92