Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 375396..376036 Replicon plasmid unnamed1
Accession NZ_CP017750
Organism Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D9HCW7
Locus tag BKK79_RS37790 Protein ID WP_071040774.1
Coordinates 375396..375797 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D9HCW3
Locus tag BKK79_RS37795 Protein ID WP_071040775.1
Coordinates 375794..376036 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK79_RS39865 371471..371941 + 471 Protein_355 cupin domain-containing protein -
BKK79_RS37775 372381..373589 + 1209 WP_011517431.1 IS4-like element ISBvi1 family transposase -
BKK79_RS37780 373859..374083 - 225 WP_071040772.1 hypothetical protein -
BKK79_RS37785 374064..374627 - 564 WP_071040773.1 hypothetical protein -
BKK79_RS37790 375396..375797 - 402 WP_071040774.1 type II toxin-antitoxin system VapC family toxin Toxin
BKK79_RS37795 375794..376036 - 243 WP_071040775.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKK79_RS37800 376571..377656 + 1086 WP_071040776.1 HNH endonuclease -
BKK79_RS37805 377725..379899 + 2175 WP_071040777.1 tyrosine-type recombinase/integrase -
BKK79_RS37810 380191..380853 - 663 WP_071040867.1 RES family NAD+ phosphorylase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..544877 544877
- flank IS/Tn - - 372390..373589 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-121)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 134 a.a.        Molecular weight: 14674.72 Da        Isoelectric Point: 6.3759

>T68095 WP_071040774.1 NZ_CP017750:c375797-375396 [Cupriavidus sp. USMAA2-4]
MKYLLDANAVIALLKGEPTFLEHLRAHLPTDFGLPAIVAHELYYGAYKSQRAAANLGRIEALQFEVIPFDAEDAQHASEI
RAQLAGAGTPIGPYDVLIAGQARARNLVLVTHNVREFARVPQLRVEDWHGEAH

Download         Length: 402 bp

>T68095 NZ_CP017750:c375797-375396 [Cupriavidus sp. USMAA2-4]
ATGAAATACCTGCTCGACGCCAACGCGGTGATCGCGCTGCTCAAGGGCGAGCCCACCTTTCTCGAGCACCTGCGTGCACA
CTTGCCGACCGATTTTGGCTTACCGGCGATCGTCGCCCACGAGCTTTACTATGGCGCCTACAAGAGCCAGCGCGCGGCCG
CCAACCTGGGCCGCATCGAGGCGCTGCAGTTTGAAGTGATACCGTTTGATGCGGAGGACGCCCAGCACGCCAGTGAGATT
CGCGCACAGTTGGCAGGCGCTGGCACGCCGATCGGCCCCTACGACGTGCTGATTGCTGGGCAGGCGAGGGCGCGCAACTT
GGTACTGGTAACCCACAACGTGCGCGAGTTTGCCCGCGTGCCCCAACTGCGCGTCGAAGACTGGCATGGCGAGGCTCACT
GA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8895.07 Da        Isoelectric Point: 4.7483

>AT68095 WP_071040775.1 NZ_CP017750:c376036-375794 [Cupriavidus sp. USMAA2-4]
METAKIFRHGGSQAVRLPKDFRFDSTEVRIRRHGAAVILEPVPQDWAWLAPLVGPLDADFEAAALAQPDAQERPGLDVFE

Download         Length: 243 bp

>AT68095 NZ_CP017750:c376036-375794 [Cupriavidus sp. USMAA2-4]
ATGGAAACGGCCAAGATCTTTCGCCACGGGGGCTCGCAGGCCGTGCGCCTGCCCAAGGACTTCCGTTTTGACAGCACCGA
GGTGCGCATCCGCCGGCACGGCGCCGCCGTCATCCTCGAACCCGTGCCGCAGGACTGGGCGTGGCTCGCGCCGCTGGTCG
GCCCGCTCGATGCCGACTTTGAGGCCGCCGCGCTTGCCCAGCCCGACGCACAGGAGCGCCCCGGCCTGGACGTGTTCGAA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1D9HCW7


Antitoxin

Source ID Structure
AlphaFold DB A0A1D9HCW3

References