Detailed information of TA system TA068094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 167762..168455
• Replicon: plasmid unnamed1
• Accession: NZ_CP017750
• Organism: Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A1D9HD98
• Locus tag: BKK79_RS36905
• Protein ID: WP_071040659.1
• Coordinates: 168078..168455 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A1D9HD92
• Locus tag: BKK79_RS36900
• Protein ID: WP_071040658.1
• Coordinates: 167762..168097 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK79_RS36865	163624..164454	+	831	WP_084085758.1	GIY-YIG nuclease family protein	-
BKK79_RS36870	164514..165383	-	870	Protein_161	IS5 family transposase	-
BKK79_RS36875	165783..166154	-	372	WP_071040654.1	hypothetical protein	-
BKK79_RS36880	166151..166651	-	501	WP_071040855.1	transposase	-
BKK79_RS36885	166648..166974	-	327	WP_071040655.1	hypothetical protein	-
BKK79_RS36890	166998..167393	-	396	WP_071040656.1	hypothetical protein	-
BKK79_RS36895	167390..167749	-	360	WP_071040657.1	transposase	-
BKK79_RS36900	167762..168097	-	336	WP_071040658.1	helix-turn-helix domain-containing protein	Antitoxin
BKK79_RS36905	168078..168455	-	378	WP_071040659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKK79_RS36910	168647..169240	+	594	WP_071040660.1	recombinase family protein	-
BKK79_RS36915	169237..172266	+	3030	WP_071040661.1	Tn3 family transposase	-
BKK79_RS36920	172263..172829	+	567	WP_071040662.1	hypothetical protein	-
BKK79_RS36925	172887..173414	-	528	WP_071040663.1	YbjN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..544877	544877
	-	inside	IScluster/Tn	-	-	164514..172266	7752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13758.90 Da
• Isoelectric Point: 8.8594

>T68094 WP_071040659.1 NZ_CP017750:c168455-168078 [Cupriavidus sp. USMAA2-4]
MVDKEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEALAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T68094 NZ_CP017750:c168455-168078 [Cupriavidus sp. USMAA2-4]
ATGGTCGATAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCTACAAGGATCTGATGGCGTTGCCGCCTGACGTGCGTCG
GCGTTTCGGTTACGCGCTGTCGCTGGCCCAGATGGGCGACCAGGACGACGCGGCGAAGGTGCTCAAGGGCTTCGGCGGGG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACGTATCGGGCGGTCTACACGGTGAAGTTCGCGGAAGCGGTG
TTTGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCCAAGGTGGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGCATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12154.33 Da
• Isoelectric Point: 10.3419

>AT68094 WP_071040658.1 NZ_CP017750:c168097-167762 [Cupriavidus sp. USMAA2-4]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVIEIRKAMRALGLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT68094 NZ_CP017750:c168097-167762 [Cupriavidus sp. USMAA2-4]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAACGCAGCTCGGGCAACGTCTTTGCCGATCTGGGACTGCCCGATGC
CGAGAAACTGAAGATCAAGACCGGCCTGGTGATCGAGATCAGGAAGGCGATGCGGGCCCTTGGCCTGACACAACAGGCGG
CGGCCAAGCGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCACGGCGATTTCACCAATCTGTCCGAACGCAAG
CTGATGGACTGCCTGAACCGTCTCGGCTACGACATCGAAATCAAGGTGCGGCCGGCGGCCGAGCCAGTCGGGCATCTGAC
GCTCGCCACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1D9HD98

Antitoxin

• AlphaFold DB: A0A1D9HD92