Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-MazE
Location 1667673..1668268 Replicon chromosome
Accession NZ_CP017749
Organism Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)


Gene name chpB Uniprot ID A0A1D9H6T2
Locus tag BKK79_RS27515 Protein ID WP_071039392.1
Coordinates 1667918..1668268 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A1D9H6K6
Locus tag BKK79_RS27510 Protein ID WP_071039391.1
Coordinates 1667673..1667924 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK79_RS27490 1662992..1663948 + 957 WP_071039388.1 LysR family transcriptional regulator -
BKK79_RS39380 1664301..1664858 - 558 WP_084085225.1 RES family NAD+ phosphorylase -
BKK79_RS27500 1665533..1666771 - 1239 WP_071039389.1 type II toxin-antitoxin system HipA family toxin -
BKK79_RS27505 1666768..1667130 - 363 WP_071039390.1 helix-turn-helix transcriptional regulator -
BKK79_RS27510 1667673..1667924 + 252 WP_071039391.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKK79_RS27515 1667918..1668268 + 351 WP_071039392.1 type II toxin-antitoxin system ChpB family toxin Toxin
BKK79_RS40610 1668234..1668356 + 123 WP_157128530.1 DUF1484 family protein -
BKK79_RS27520 1668430..1668837 - 408 WP_071039393.1 cupin domain-containing protein -
BKK79_RS27525 1668918..1669745 - 828 WP_071039394.1 AraC family transcriptional regulator -
BKK79_RS27530 1669848..1670264 - 417 WP_071039395.1 NUDIX domain-containing protein -
BKK79_RS40615 1670388..1670837 - 450 WP_157128531.1 hypothetical protein -
BKK79_RS40620 1670897..1671715 - 819 WP_157128532.1 hypothetical protein -
BKK79_RS40625 1671838..1672656 - 819 WP_157128533.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1641893..1673800 31907


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-116)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12344.15 Da        Isoelectric Point: 6.3482

>T68091 WP_071039392.1 NZ_CP017749:1667918-1668268 [Cupriavidus sp. USMAA2-4]
MVRRAKFDRGDIVRVSLNPTEGREQQGDFRPALVLSPAAFNALGVALVAPITQGGDFARFAGFAVPLSGAGTETQGVALV
NMVRTLDLEARGARKIERAPAEVVEDALARLQTILD

Download         Length: 351 bp

>T68091 NZ_CP017749:1667918-1668268 [Cupriavidus sp. USMAA2-4]
ATGGTGAGGCGCGCGAAGTTCGACCGGGGCGACATTGTGCGGGTGAGCCTGAACCCAACGGAGGGGCGTGAGCAGCAAGG
GGATTTCCGTCCCGCGCTCGTGCTGTCACCGGCAGCATTCAACGCTCTGGGTGTTGCCCTGGTGGCACCGATTACGCAGG
GCGGCGATTTCGCTCGATTCGCCGGATTTGCCGTACCGTTGTCCGGGGCGGGCACGGAGACTCAAGGCGTCGCGCTGGTG
AACATGGTGCGCACTCTGGATCTTGAGGCTCGCGGTGCGAGGAAGATCGAACGGGCACCTGCCGAGGTTGTGGAGGATGC
GCTCGCCCGTCTTCAAACCATCCTGGATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9141.53 Da        Isoelectric Point: 5.8125

>AT68091 WP_071039391.1 NZ_CP017749:1667673-1667924 [Cupriavidus sp. USMAA2-4]
MELSIQKWGNSAAVRLPTTLLEQLKVALGDKLSVEVRPDGVMLKPARHKYSLDELLAQCDSKAPTPEGLDDWSGAKPVGR
EVW

Download         Length: 252 bp

>AT68091 NZ_CP017749:1667673-1667924 [Cupriavidus sp. USMAA2-4]
ATGGAACTGTCGATTCAGAAATGGGGTAACAGCGCGGCCGTGCGCCTGCCCACGACCTTGCTTGAACAACTCAAGGTGGC
GTTGGGCGACAAACTGTCCGTGGAGGTGCGTCCGGATGGTGTCATGCTTAAACCAGCCAGGCACAAGTATTCACTCGATG
AACTGCTTGCGCAGTGCGACTCCAAGGCGCCGACGCCTGAAGGGCTGGATGATTGGAGCGGGGCTAAGCCGGTTGGGCGC
GAAGTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9H6T2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H6K6

References