Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1499903..1500939 Replicon chromosome
Accession NZ_CP017749
Organism Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A1D9H6F9
Locus tag BKK79_RS26730 Protein ID WP_071039288.1
Coordinates 1499903..1500478 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A1D9H662
Locus tag BKK79_RS26735 Protein ID WP_071017164.1
Coordinates 1500475..1500939 (-) Length 155 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK79_RS26700 1494943..1495872 - 930 WP_071039284.1 glyoxylate/hydroxypyruvate reductase A -
BKK79_RS26705 1496042..1496824 - 783 WP_071017182.1 class II aldolase/adducin family protein -
BKK79_RS26710 1496884..1497693 + 810 WP_083384304.1 Crp/Fnr family transcriptional regulator -
BKK79_RS26715 1497926..1498198 + 273 WP_157128520.1 hypothetical protein -
BKK79_RS26720 1498230..1498916 - 687 WP_071039286.1 signal peptidase I -
BKK79_RS26725 1499097..1499714 + 618 WP_071039287.1 J domain-containing protein -
BKK79_RS26730 1499903..1500478 - 576 WP_071039288.1 PIN domain-containing protein Toxin
BKK79_RS26735 1500475..1500939 - 465 WP_071017164.1 helix-turn-helix domain-containing protein Antitoxin
BKK79_RS26740 1501148..1502296 - 1149 WP_157128521.1 acyltransferase -
BKK79_RS26745 1502304..1503158 - 855 WP_071039289.1 class D beta-lactamase -
BKK79_RS26750 1503245..1504987 - 1743 WP_157128522.1 serine hydrolase -
BKK79_RS26755 1504984..1505373 - 390 WP_071017158.1 BlaI/MecI/CopY family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1499903..1509922 10019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(10-115)

Antitoxin

Helix-turn-helix domain, group 17

(83-131)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21833.95 Da        Isoelectric Point: 5.6305

>T68090 WP_071039288.1 NZ_CP017749:c1500478-1499903 [Cupriavidus sp. USMAA2-4]
MRHSPFTALYDACVLYPAPLRDFLMWLGLSGRFRARWSPQIHDEWKRNLLANREDLTREQLDRTSDLMDRAIPDALVLDY
EALAVELRLPDPDDRHVLAAAIRCGASVIVTFNRKDFPDDALAPFGIEARHPDEFIDNLFDLDAAAVVAAARQQRAQLKH
PPMDVDRYLDVLQRQGLLQTVKGLGQYRSIL

Download         Length: 576 bp

>T68090 NZ_CP017749:c1500478-1499903 [Cupriavidus sp. USMAA2-4]
ATGAGGCATTCGCCATTCACAGCCCTGTATGACGCTTGCGTGCTGTATCCAGCGCCACTGCGCGACTTTCTCATGTGGCT
GGGCCTGTCGGGGCGGTTCCGTGCACGGTGGAGTCCGCAGATCCACGACGAATGGAAGCGCAATCTGCTGGCTAACCGCG
AAGACCTGACGCGCGAGCAACTGGATCGCACATCGGACCTGATGGATCGCGCTATCCCTGATGCCTTGGTCCTTGACTAC
GAGGCACTTGCCGTCGAACTACGGTTACCGGACCCAGACGACCGGCACGTACTCGCCGCCGCCATTCGTTGCGGCGCCAG
CGTCATCGTCACCTTCAACCGGAAAGACTTTCCGGACGACGCATTGGCACCGTTCGGCATCGAGGCCCGGCACCCCGACG
AATTCATCGACAACCTGTTCGACCTGGATGCGGCCGCTGTCGTTGCCGCCGCCCGGCAGCAGCGCGCGCAGCTCAAGCAT
CCGCCCATGGATGTCGACCGCTACCTCGACGTGCTGCAACGCCAGGGCCTGCTGCAAACCGTCAAGGGCCTGGGCCAATA
CCGTTCCATCCTGTAG

Antitoxin


Download         Length: 155 a.a.        Molecular weight: 16946.27 Da        Isoelectric Point: 6.4928

>AT68090 WP_071017164.1 NZ_CP017749:c1500939-1500475 [Cupriavidus sp. USMAA2-4]
VTAAYSRVTLPVEREVQAAVQGQRALAAYLATQVETQRIQIFDDKNQAHQVELPTSALRLLVDILAELAGGNAVKVVPVH
AELTTQEAADLLNVSRPHLVKLLEDGALPFHRAGKHRRVRFSDLMQFKAARDQASEQAMAELAHQAQVSDMGYE

Download         Length: 465 bp

>AT68090 NZ_CP017749:c1500939-1500475 [Cupriavidus sp. USMAA2-4]
GTGACCGCCGCCTATTCGAGAGTAACGCTACCCGTGGAACGGGAAGTGCAGGCTGCCGTGCAAGGTCAACGCGCCTTGGC
GGCTTATCTGGCCACCCAGGTCGAGACCCAGCGCATCCAGATCTTCGATGACAAGAATCAAGCTCATCAGGTGGAGCTGC
CAACATCGGCGCTCCGCCTGCTGGTGGACATCCTGGCCGAACTGGCCGGCGGCAATGCCGTCAAGGTCGTGCCTGTTCAT
GCCGAGTTGACCACGCAGGAAGCGGCCGACCTGCTCAACGTCTCCCGGCCGCACCTGGTCAAGTTGCTGGAAGACGGCGC
GCTGCCGTTCCATCGGGCTGGCAAGCACCGCCGCGTGCGCTTTTCGGACCTGATGCAGTTCAAGGCCGCGCGCGATCAGG
CCAGTGAGCAAGCCATGGCCGAGTTGGCGCATCAAGCCCAGGTGTCTGACATGGGTTACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9H6F9

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H662

References