Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2412475..2414087 Replicon chromosome
Accession NZ_CP017748
Organism Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)


Gene name hipA Uniprot ID A0A1D9GX78
Locus tag BKK79_RS10635 Protein ID WP_071037679.1
Coordinates 2412475..2413815 (-) Length 447 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1D9H2X1
Locus tag BKK79_RS10640 Protein ID WP_071015926.1
Coordinates 2413821..2414087 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKK79_RS10605 2407999..2408928 + 930 WP_071037677.1 alpha/beta hydrolase -
BKK79_RS10610 2409051..2409659 + 609 WP_198173327.1 lecithin retinol acyltransferase family protein -
BKK79_RS10615 2409656..2410177 + 522 WP_084084665.1 dethiobiotin synthetase -
BKK79_RS10620 2410298..2410900 + 603 WP_071037678.1 RNA polymerase sigma factor -
BKK79_RS10625 2410890..2411756 + 867 WP_157128418.1 anti-sigma factor -
BKK79_RS10630 2411916..2412152 + 237 WP_071010575.1 hypothetical protein -
BKK79_RS40290 2412145..2412384 + 240 WP_157128419.1 hypothetical protein -
BKK79_RS10635 2412475..2413815 - 1341 WP_071037679.1 type II toxin-antitoxin system HipA family toxin Toxin
BKK79_RS10640 2413821..2414087 - 267 WP_071015926.1 helix-turn-helix domain-containing protein Antitoxin
BKK79_RS10645 2414277..2414552 - 276 WP_071010573.1 muconolactone Delta-isomerase -
BKK79_RS10650 2414673..2415011 - 339 WP_071010572.1 4-methylmuconolactone methylisomerase -
BKK79_RS10655 2415105..2416391 - 1287 WP_071037680.1 MFS transporter -
BKK79_RS10660 2416574..2417236 - 663 WP_071010570.1 3-oxoacid CoA-transferase subunit B -
BKK79_RS10665 2417242..2417985 - 744 WP_071010569.1 3-oxoacid CoA-transferase subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(157-386)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-67)


Sequences

Toxin        


Download         Length: 447 a.a.        Molecular weight: 49163.36 Da        Isoelectric Point: 8.4868

>T68087 WP_071037679.1 NZ_CP017748:c2413815-2412475 [Cupriavidus sp. USMAA2-4]
MGRRSRTRSLGLWMNGVYVGNWQFQPQHGDVLQYAEAWLSAPEGRPLSLSLPFTPGNAAHRGDAVRAYFENLLPDSREIR
DRIARRFRTETTDAFDLLAQVGRDCVGALQLLPDGMAPTGVATIDGEPLNEAQMARALRGAVMQQPPAEADDGEFRISIA
GAQEKTAFLWADGQWLRPRGSTPTSHIFKLPMGLVGNMQLDLGESVENEWLCAQILAAYGMPVAACRPLMFENQKVLCVE
RFDRMWSKGSKPWLIRLPQEDMCQATATPPYRKHEADGGPGIDRIMRLLDGSMHREADRLRFFQAQVLFWMLCAPDGHAK
NFSLALRPGGAYQLTPLYDVMSAYPLLGEGPGKLSPHKVRLAMAVRSSNAHWRVRDIRRRHWLELGRRHAIVTPEGGDAE
SVVNGLAASTPAVVTRVRSLLPAGFPSALADTILGGLQAAADKLAA

Download         Length: 1341 bp

>T68087 NZ_CP017748:c2413815-2412475 [Cupriavidus sp. USMAA2-4]
ATGGGGCGCCGCTCTCGCACGCGCAGCCTCGGACTCTGGATGAACGGCGTCTATGTCGGCAACTGGCAGTTCCAGCCGCA
ACACGGGGACGTACTGCAATATGCGGAGGCCTGGCTTTCGGCGCCCGAAGGGCGTCCGCTGTCCTTGTCGCTGCCGTTCA
CGCCGGGCAACGCCGCGCATCGGGGTGACGCCGTGCGCGCCTACTTCGAAAACCTGCTGCCCGACAGTCGGGAGATACGC
GATCGCATTGCACGGCGTTTCCGCACGGAAACGACCGATGCCTTCGATCTGTTGGCACAAGTCGGGCGCGACTGTGTGGG
CGCACTGCAATTGCTGCCGGACGGCATGGCGCCGACAGGCGTCGCCACGATCGATGGGGAACCACTGAACGAAGCGCAGA
TGGCGAGGGCATTGCGCGGAGCGGTCATGCAGCAGCCGCCAGCCGAGGCCGATGATGGTGAGTTCCGCATATCGATCGCC
GGCGCCCAGGAGAAGACTGCTTTCCTGTGGGCGGACGGTCAATGGTTGCGCCCCAGAGGCTCCACGCCCACCAGTCATAT
CTTCAAGCTCCCCATGGGACTGGTCGGCAATATGCAACTGGACCTGGGCGAATCGGTCGAGAATGAATGGCTGTGCGCAC
AGATCCTCGCGGCCTATGGCATGCCCGTGGCCGCTTGCCGGCCGCTCATGTTCGAGAACCAGAAGGTGCTCTGTGTCGAA
CGCTTCGATCGCATGTGGTCGAAGGGAAGCAAGCCCTGGCTCATCCGCCTGCCTCAGGAGGACATGTGCCAGGCCACCGC
CACGCCGCCATATCGGAAGCACGAAGCCGATGGTGGCCCGGGGATCGACCGCATCATGCGTCTGCTCGATGGCTCGATGC
ATCGTGAGGCGGATCGCCTGCGCTTTTTCCAGGCACAGGTTCTGTTCTGGATGCTGTGCGCCCCCGACGGACATGCCAAG
AATTTCAGCTTGGCGTTGCGTCCTGGTGGCGCCTACCAGCTGACACCCCTATACGATGTGATGTCGGCCTATCCACTGCT
TGGCGAAGGACCAGGGAAACTCTCGCCGCACAAGGTCCGCCTGGCGATGGCGGTCCGTTCCAGCAACGCACATTGGCGAG
TACGGGACATCCGGCGGCGGCACTGGCTGGAACTGGGGCGCCGCCACGCCATCGTCACGCCGGAGGGCGGCGACGCGGAG
TCCGTGGTCAACGGTCTCGCAGCAAGCACGCCTGCGGTAGTCACCCGCGTGCGCAGCCTGTTGCCAGCGGGGTTCCCTTC
GGCACTGGCCGACACCATCCTTGGTGGATTGCAGGCGGCGGCGGACAAGCTTGCGGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9535.01 Da        Isoelectric Point: 11.0917

>AT68087 WP_071015926.1 NZ_CP017748:c2414087-2413821 [Cupriavidus sp. USMAA2-4]
MREFTVRTPEQLPAMLRGFRKQAGLTQAELAARLGVRQQTLSGLERNAESVSAGRLLTLLSVLGVELVLRQADAAAQAAP
PPTDLPQW

Download         Length: 267 bp

>AT68087 NZ_CP017748:c2414087-2413821 [Cupriavidus sp. USMAA2-4]
ATGCGCGAATTCACGGTCCGCACGCCCGAGCAACTCCCAGCCATGCTCCGAGGCTTCCGCAAGCAAGCCGGCCTGACCCA
GGCCGAACTGGCTGCCCGTCTGGGCGTACGCCAGCAGACATTGTCGGGGCTGGAGCGCAACGCGGAAAGCGTCAGCGCCG
GGCGCCTGTTGACGCTGCTGTCGGTATTGGGTGTCGAACTCGTGCTTCGGCAAGCGGACGCTGCCGCGCAGGCCGCGCCC
CCGCCAACCGATCTTCCTCAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9GX78

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9H2X1

References