Detailed information of TA system TA068086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1547496..1548080
• Replicon: chromosome
• Accession: NZ_CP017748
• Organism: Cupriavidus sp. USMAA2-4 isolate pure

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BKK79_RS38895
• Protein ID: WP_084084555.1
• Coordinates: 1547496..1547678 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1D9GV91
• Locus tag: BKK79_RS06790
• Protein ID: WP_071011396.1
• Coordinates: 1547688..1548080 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKK79_RS06765	1542681..1543064	+	384	WP_156811135.1	hypothetical protein	-
BKK79_RS06770	1543176..1543580	+	405	WP_084084862.1	hypothetical protein	-
BKK79_RS06775	1543758..1544075	-	318	WP_071038441.1	multidrug efflux SMR transporter	-
BKK79_RS06780	1544328..1545176	+	849	WP_071037411.1	hypothetical protein	-
BKK79_RS06785	1545699..1546754	-	1056	WP_084084553.1	chitinase	-
BKK79_RS38895	1547496..1547678	+	183	WP_084084555.1	type II toxin-antitoxin system HicA family toxin	Toxin
BKK79_RS06790	1547688..1548080	+	393	WP_071011396.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BKK79_RS38900	1548197..1548628	-	432	WP_157128404.1	hypothetical protein	-
BKK79_RS06800	1548760..1549956	-	1197	WP_071038442.1	DUF4382 domain-containing protein	-
BKK79_RS06805	1550523..1551401	+	879	Protein_1389	LysR family transcriptional regulator	-
BKK79_RS06810	1551604..1552401	-	798	WP_071038443.1	2-keto-4-pentenoate hydratase	-
BKK79_RS06815	1552460..1552753	-	294	WP_071011393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	bplB	1505006..1548514	43508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6815.87 Da
• Isoelectric Point: 11.2176

>T68086 WP_084084555.1 NZ_CP017748:1547496-1547678 [Cupriavidus sp. USMAA2-4]
MHSADLIRQLKADGWQLVYTVGSHHQFRYPTTPGKVTVPHPNKDLPIGTVRRLQKQAGLR

Nucleotide

• Length: 183 bp

>T68086 NZ_CP017748:1547496-1547678 [Cupriavidus sp. USMAA2-4]
ATGCATAGCGCAGACCTGATCCGGCAACTCAAGGCGGATGGCTGGCAACTCGTCTACACCGTGGGTTCCCACCATCAGTT
CAGGTACCCGACGACGCCGGGAAAGGTAACGGTCCCGCATCCGAACAAGGATCTCCCGATAGGCACGGTGCGCAGACTCC
AGAAACAGGCTGGCTTGAGGTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14353.26 Da
• Isoelectric Point: 4.9323

>AT68086 WP_071011396.1 NZ_CP017748:1547688-1548080 [Cupriavidus sp. USMAA2-4]
MLYPLYVHVGDARHAHGVSFPDFPGCFAAADDWEDLPAAVQEAAEAHFAGSETPIPRPTPLESLTASPEFEGGVWMLFDI
DLSKLRSKAVRLNISLPERLLQQIDAAARQRRLSRSAFLALAAEHEMERA

Nucleotide

• Length: 393 bp

>AT68086 NZ_CP017748:1547688-1548080 [Cupriavidus sp. USMAA2-4]
ATGCTCTATCCCCTGTATGTCCACGTGGGAGACGCCAGGCACGCGCACGGCGTCTCATTCCCCGACTTCCCGGGCTGCTT
TGCCGCGGCAGACGACTGGGAAGATCTGCCCGCCGCCGTGCAGGAGGCCGCCGAAGCCCATTTCGCCGGCAGCGAGACAC
CCATTCCCCGGCCAACGCCGTTGGAATCCCTGACCGCATCGCCCGAGTTCGAAGGCGGCGTCTGGATGCTGTTCGACATC
GATCTGTCCAAGCTGAGATCCAAGGCGGTGCGGCTGAATATCAGCCTGCCGGAACGGCTTCTTCAGCAGATCGACGCGGC
CGCCAGGCAGCGGCGCCTGTCGCGCTCGGCCTTCCTCGCCCTGGCGGCGGAGCACGAGATGGAGCGAGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1D9GV91