Detailed information of TA system TA068085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2376320..2376957
• Replicon: chromosome
• Accession: NZ_CP017747
• Organism: Bacillus velezensis strain SYBC H47

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BI197_RS11430
• Protein ID: WP_003156187.1
• Coordinates: 2376320..2376670 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BI197_RS11435
• Protein ID: WP_003156188.1
• Coordinates: 2376676..2376957 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI197_RS11390	2371360..2371962	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
BI197_RS11395	2371962..2372750	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BI197_RS11400	2372716..2373198	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
BI197_RS11405	2373195..2373524	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BI197_RS11410	2373588..2374595	-	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
BI197_RS11415	2374607..2375008	-	402	WP_003156178.1	anti-sigma regulatory factor	-
BI197_RS11420	2375011..2375376	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BI197_RS11425	2375381..2376202	-	822	WP_003156182.1	STAS domain-containing protein	-
BI197_RS11430	2376320..2376670	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BI197_RS11435	2376676..2376957	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BI197_RS11440	2377077..2378246	-	1170	WP_071392103.1	alanine racemase	-
BI197_RS11445	2378363..2379370	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
BI197_RS11450	2379535..2379900	-	366	WP_071392104.1	holo-ACP synthase	-
BI197_RS11455	2379993..2380592	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T68085 WP_003156187.1 NZ_CP017747:c2376670-2376320 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T68085 NZ_CP017747:c2376670-2376320 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT68085 WP_003156188.1 NZ_CP017747:c2376957-2376676 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT68085 NZ_CP017747:c2376957-2376676 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5