Detailed information of TA system TA068082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5326..5851
• Replicon: plasmid pSARA13
• Accession: NZ_CP017729
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SARA13 strain SGSC 2193

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: SEETRA13_RS23330
• Protein ID: WP_001159863.1
• Coordinates: 5546..5851 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: SEETRA13_RS23325
• Protein ID: WP_000813641.1
• Coordinates: 5326..5544 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SEETRA13_RS23300	613..994	+	382	Protein_0	YgiW/YdeI family stress tolerance OB fold protein	-
SEETRA13_RS25665	1163..1607	+	445	Protein_1	site-specific integrase	-
SEETRA13_RS23310	1866..2855	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
SEETRA13_RS23315	3349..3645	-	297	WP_001687482.1	hypothetical protein	-
SEETRA13_RS23320	4129..4650	-	522	WP_010999942.1	hypothetical protein	-
SEETRA13_RS23325	5326..5544	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
SEETRA13_RS23330	5546..5851	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
SEETRA13_RS23335	5853..6143	+	291	WP_001266176.1	hypothetical protein	-
SEETRA13_RS23340	6140..6661	+	522	WP_000198608.1	hypothetical protein	-
SEETRA13_RS23345	6696..7478	+	783	WP_000082169.1	site-specific integrase	-
SEETRA13_RS23350	7487..8200	+	714	Protein_10	EAL domain-containing protein	-
SEETRA13_RS23355	8204..8713	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
SEETRA13_RS23360	8707..9192	+	486	WP_000905606.1	membrane protein	-
SEETRA13_RS25670	9469..9756	-	288	WP_071530243.1	hypothetical protein	-
SEETRA13_RS23370	9912..10472	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93826	93826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T68082 WP_001159863.1 NZ_CP017729:5546-5851 [Salmonella enterica subsp. enterica serovar Typhimurium str. SARA13]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T68082 NZ_CP017729:5546-5851 [Salmonella enterica subsp. enterica serovar Typhimurium str. SARA13]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT68082 WP_000813641.1 NZ_CP017729:5326-5544 [Salmonella enterica subsp. enterica serovar Typhimurium str. SARA13]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT68082 NZ_CP017729:5326-5544 [Salmonella enterica subsp. enterica serovar Typhimurium str. SARA13]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6