Detailed information of TA system TA068070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2551150..2551782
• Replicon: chromosome
• Accession: NZ_CP017705
• Organism: Brevibacillus laterosporus DSM 25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: BrL25_RS12270
• Protein ID: WP_003333534.1
• Coordinates: 2551432..2551782 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BrL25_RS12265
• Protein ID: WP_173645602.1
• Coordinates: 2551150..2551428 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BrL25_RS12240	2546850..2547182	+	333	WP_018673808.1	metalloregulator ArsR/SmtB family transcription factor	-
BrL25_RS12245	2547390..2547722	+	333	WP_018673807.1	gamma-type small acid-soluble spore protein	-
BrL25_RS12250	2548070..2549101	+	1032	WP_018673806.1	DUF4367 domain-containing protein	-
BrL25_RS12255	2549334..2549777	+	444	WP_018673805.1	hypothetical protein	-
BrL25_RS12260	2549781..2550986	+	1206	WP_018673804.1	alanine racemase	-
BrL25_RS12265	2551150..2551428	+	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
BrL25_RS12270	2551432..2551782	+	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BrL25_RS12275	2552012..2554171	+	2160	WP_018673803.1	RNA-binding transcriptional accessory protein	-
BrL25_RS12280	2554249..2554374	-	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
BrL25_RS12285	2554490..2554825	-	336	WP_018673802.1	hypothetical protein	-
BrL25_RS12290	2554946..2555410	+	465	WP_018673801.1	SprT family protein	-
BrL25_RS12295	2555476..2555946	+	471	WP_018673800.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
BrL25_RS12300	2555927..2556640	+	714	WP_018673799.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T68070 WP_003333534.1 NZ_CP017705:2551432-2551782 [Brevibacillus laterosporus DSM 25]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T68070 NZ_CP017705:2551432-2551782 [Brevibacillus laterosporus DSM 25]
GTGAATGTTAAACGTGGCGATGTATTTTTCGCGGACCTTTCCCCTGTGGTTGGCTCCGAGCAAGGTGGAGTTAGACCGGT
ATTAGTTATACAAAACGATATTGGAAATCGATTTAGTCCGACGGTTATCGTCGCTGCGATCACTGCTCAGATTCAAAAGG
CAAAGCTGCCTACGCATGTGGAAATCGATGCAAAAACATATGGGTTTGATCGTGACTCTGTCATCTTATTGGAACAAATA
AGAACCATTGATAAGCAAAGGTTGACTGATAAAATCACTCATCTAGACGATGAGATGATGGAAAGAGTGAATGAATCACT
TCAGATCAGCCTCGGATTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT68070 WP_173645602.1 NZ_CP017705:2551150-2551428 [Brevibacillus laterosporus DSM 25]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT68070 NZ_CP017705:2551150-2551428 [Brevibacillus laterosporus DSM 25]
TTGTCTAAAAGCAATACAAAACGTATCATGATCAGTTTGCCGCAACATTTATTGCAAGAGGTAGACGGAGTCGTGCAAAA
AGAGAGATCAAACCGAAGTGAATTGATTCGTCAAGCGATGAGTCTCTACCTCAAGGAGCGAAAAAAACGATTTATACGAG
AAACAATGCAACGTGGCTATTTGGAAATGGCTAAAATCAATCTCAATATGGCATCGGAGGCTTTTGAAGCAGAAGAGGAA
GCCGATCTTACCTTAGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8