Detailed information of TA system TA068065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-DinJ
• Location: 532176..532701
• Replicon: chromosome
• Accession: NZ_CP017696
• Organism: Bifidobacterium asteroides DSM 20089

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: K4J095
• Locus tag: BA20089_RS01990
• Protein ID: WP_015021574.1
• Coordinates: 532176..532454 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K4IQ07
• Locus tag: BA20089_RS01995
• Protein ID: WP_015021575.1
• Coordinates: 532447..532701 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA20089_RS01965	527455..528348	+	894	WP_015021569.1	FtsQ-type POTRA domain-containing protein	-
BA20089_RS01970	528341..528841	-	501	WP_015021570.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BA20089_RS01975	529236..529754	-	519	WP_015021571.1	GNAT family N-acetyltransferase	-
BA20089_RS01980	529823..530140	-	318	WP_015021572.1	multidrug efflux SMR transporter	-
BA20089_RS01985	530416..531855	+	1440	WP_015021573.1	FtsX-like permease family protein	-
BA20089_RS01990	532176..532454	-	279	WP_015021574.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
BA20089_RS01995	532447..532701	-	255	WP_015021575.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BA20089_RS02005	533455..534426	-	972	WP_033510981.1	LysR family transcriptional regulator	-
BA20089_RS02010	534629..536251	-	1623	WP_015021577.1	NAD-dependent malic enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11273.06 Da
• Isoelectric Point: 10.3456

>T68065 WP_015021574.1 NZ_CP017696:c532454-532176 [Bifidobacterium asteroides DSM 20089]
MYKFAYTQKFLRDVKKIKRKHFDLSRLDQVLDDLADYNASLLKRKYRNHRLKGHWSKYEELHVDADWLLVYKRDKNRMIL
TLMTTGSHDQTL

Nucleotide

• Length: 279 bp

>T68065 NZ_CP017696:c532454-532176 [Bifidobacterium asteroides DSM 20089]
ATGTATAAGTTTGCATATACGCAAAAATTCCTGCGTGATGTCAAGAAAATCAAAAGGAAGCATTTCGACTTGAGCAGGCT
TGATCAGGTACTTGACGACTTGGCTGACTACAATGCATCACTGCTTAAACGAAAATACCGAAATCACCGATTGAAAGGAC
ACTGGTCTAAATACGAGGAGCTGCATGTCGACGCGGACTGGCTGCTCGTGTACAAGCGCGATAAGAATCGAATGATTCTT
ACCTTGATGACCACCGGGAGCCATGACCAAACACTGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9561.82 Da
• Isoelectric Point: 5.1312

>AT68065 WP_015021575.1 NZ_CP017696:c532701-532447 [Bifidobacterium asteroides DSM 20089]
MKETARIQIRTSEDMKRRASQLFEDLGLDLGTAINMFLSQSLREGGLPFRSQLSKFDREMEEAEASPVTHAGDVENMKDI
IHHV

Nucleotide

• Length: 255 bp

>AT68065 NZ_CP017696:c532701-532447 [Bifidobacterium asteroides DSM 20089]
ATGAAAGAGACAGCGAGAATTCAAATTCGAACCAGCGAGGATATGAAGCGTCGAGCCTCACAGCTTTTCGAAGATTTAGG
CCTTGATCTCGGCACGGCTATTAACATGTTTCTCTCTCAGTCGCTGCGCGAAGGAGGACTTCCATTCCGCTCCCAACTTT
CCAAATTTGACAGGGAAATGGAGGAAGCCGAGGCTTCACCGGTCACTCACGCGGGTGATGTAGAGAACATGAAAGACATC
ATCCATCATGTATAA

Structures

Toxin

• AlphaFold DB: K4J095

Antitoxin

• AlphaFold DB: K4IQ07