Detailed information of TA system TA068064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1216019..1216616
• Replicon: chromosome
• Accession: NZ_CP017695
• Organism: Bifidobacterium pseudolongum subsp. globosum DSM 20092

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BP20092_RS04870
• Protein ID: WP_080699166.1
• Coordinates: 1216019..1216321 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N3QV32
• Locus tag: BP20092_RS04875
• Protein ID: WP_026643099.1
• Coordinates: 1216323..1216616 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BP20092_RS04840	1211854..1212672	+	819	WP_026643103.1	dihydroorotate dehydrogenase electron transfer subunit	-
BP20092_RS04845	1212657..1213646	+	990	WP_051591923.1	dihydroorotate dehydrogenase	-
BP20092_RS04850	1213686..1214381	+	696	WP_026643101.1	orotate phosphoribosyltransferase	-
BP20092_RS04855	1214415..1215233	+	819	WP_080699168.1	hypothetical protein	-
BP20092_RS04865	1215481..1215908	+	428	Protein_936	GNAT family N-acetyltransferase	-
BP20092_RS04870	1216019..1216321	+	303	WP_080699166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BP20092_RS04875	1216323..1216616	+	294	WP_026643099.1	putative addiction module antidote protein	Antitoxin
BP20092_RS04880	1216738..1217082	-	345	WP_026643098.1	helix-turn-helix transcriptional regulator	-
BP20092_RS04885	1217248..1218177	-	930	WP_026643097.1	LysR family transcriptional regulator	-
BP20092_RS04890	1218211..1218852	-	642	WP_026643096.1	HAD family phosphatase	-
BP20092_RS04895	1219047..1220795	-	1749	WP_033502353.1	FAD-dependent oxidoreductase	-
BP20092_RS04900	1220822..1221385	-	564	WP_026643094.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11494.14 Da
• Isoelectric Point: 9.8170

>T68064 WP_080699166.1 NZ_CP017695:1216019-1216321 [Bifidobacterium pseudolongum subsp. globosum DSM 20092]
MEIRTTEQFDKWFRKLRDRKAKAKISARLRQCEISDHITGDHHGIGGGISEMRFHFGPGYRIYYTVDNDVLVILLIGSGK
DDQSRGIAEARKLLALYRGE

Nucleotide

• Length: 303 bp

>T68064 NZ_CP017695:1216019-1216321 [Bifidobacterium pseudolongum subsp. globosum DSM 20092]
ATGGAGATTCGTACTACTGAGCAATTCGATAAATGGTTCCGCAAGTTGCGGGACCGCAAAGCAAAAGCCAAAATCAGCGC
ACGGCTCAGACAATGCGAAATCTCCGACCATATCACTGGAGATCATCATGGCATCGGCGGTGGCATAAGTGAAATGAGAT
TCCATTTTGGACCGGGATATCGTATCTACTACACCGTTGACAATGATGTGCTTGTTATTTTACTGATCGGAAGTGGTAAA
GACGATCAGTCCAGAGGGATTGCCGAAGCGAGAAAACTTCTTGCTTTATATCGAGGAGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10542.01 Da
• Isoelectric Point: 4.5837

>AT68064 WP_026643099.1 NZ_CP017695:1216323-1216616 [Bifidobacterium pseudolongum subsp. globosum DSM 20092]
MTLKTTKWDVTEYLDNEEDIAEYLNAVIELDDPALLQAAIGDVAKAQGMTKIAKDAGLGRESLYKSLSAQGNPSFTTILK
VLHALGTRISIQPEHAI

Nucleotide

• Length: 294 bp

>AT68064 NZ_CP017695:1216323-1216616 [Bifidobacterium pseudolongum subsp. globosum DSM 20092]
ATGACGCTTAAAACCACCAAATGGGATGTTACGGAATATCTTGATAATGAAGAGGATATCGCCGAATACCTCAACGCAGT
AATCGAATTGGACGATCCTGCGTTGCTCCAAGCTGCTATTGGTGATGTTGCCAAGGCGCAGGGGATGACGAAAATCGCCA
AAGATGCAGGCCTAGGCAGAGAAAGCCTATATAAAAGTCTGAGTGCACAAGGCAATCCTTCTTTCACGACCATATTGAAA
GTACTGCATGCTCTCGGTACAAGAATCAGCATTCAACCAGAACACGCTATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N3QV32