Detailed information of TA system TA068052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2010125..2010654
• Replicon: chromosome
• Accession: NZ_CP017685
• Organism: Staphylococcus aureus strain CFSAN007835

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB525_RS10205
• Protein ID: WP_000621175.1
• Coordinates: 2010125..2010487 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AB525_RS10210
• Protein ID: WP_000948331.1
• Coordinates: 2010484..2010654 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB525_RS10180	2007104..2007874	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
AB525_RS10185	2007849..2008328	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
AB525_RS10190	2008330..2008656	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AB525_RS10195	2008775..2009776	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AB525_RS10205	2010125..2010487	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB525_RS10210	2010484..2010654	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AB525_RS10215	2010739..2011887	-	1149	WP_001281140.1	alanine racemase	-
AB525_RS10220	2011953..2012312	-	360	WP_000581199.1	holo-ACP synthase	-
AB525_RS10225	2012316..2012807	-	492	WP_001205907.1	PH domain-containing protein	-
AB525_RS10230	2012794..2014377	-	1584	WP_001294637.1	PH domain-containing protein	-
AB525_RS10235	2014370..2014849	-	480	WP_001287077.1	hypothetical protein	-
AB525_RS10240	2015057..2015617	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T68052 WP_000621175.1 NZ_CP017685:c2010487-2010125 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T68052 NZ_CP017685:c2010487-2010125 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT68052 WP_000948331.1 NZ_CP017685:c2010654-2010484 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT68052 NZ_CP017685:c2010654-2010484 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1