Detailed information of TA system TA067993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2064184..2064713
• Replicon: chromosome
• Accession: NZ_CP017679
• Organism: Staphylococcus aureus strain CFSAN007883

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB478_RS10500
• Protein ID: WP_000621175.1
• Coordinates: 2064184..2064546 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AB478_RS10505
• Protein ID: WP_000948331.1
• Coordinates: 2064543..2064713 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB478_RS10475	2061163..2061933	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
AB478_RS10480	2061908..2062387	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
AB478_RS10485	2062389..2062715	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AB478_RS10490	2062834..2063835	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AB478_RS10500	2064184..2064546	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB478_RS10505	2064543..2064713	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AB478_RS10510	2064798..2065946	-	1149	WP_001280702.1	alanine racemase	-
AB478_RS10515	2066012..2066371	-	360	WP_000581194.1	holo-ACP synthase	-
AB478_RS10520	2066375..2066866	-	492	WP_001205915.1	PH domain-containing protein	-
AB478_RS10525	2066853..2068436	-	1584	WP_001294642.1	PH domain-containing protein	-
AB478_RS10530	2068429..2068908	-	480	WP_001287083.1	hypothetical protein	-
AB478_RS10535	2069117..2069677	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T67993 WP_000621175.1 NZ_CP017679:c2064546-2064184 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T67993 NZ_CP017679:c2064546-2064184 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT67993 WP_000948331.1 NZ_CP017679:c2064713-2064543 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT67993 NZ_CP017679:c2064713-2064543 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1