Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2423205..2423841 Replicon chromosome
Accession NZ_CP017676
Organism Bacillus subtilis strain VV2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BKN48_RS12675 Protein ID WP_003156187.1
Coordinates 2423491..2423841 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BKN48_RS12670 Protein ID WP_003225183.1
Coordinates 2423205..2423486 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKN48_RS12650 2419565..2420164 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
BKN48_RS12655 2420259..2420624 + 366 WP_003234281.1 holo-ACP synthase -
BKN48_RS12660 2420790..2421806 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
BKN48_RS12665 2421920..2423089 + 1170 WP_015252766.1 alanine racemase -
BKN48_RS12670 2423205..2423486 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BKN48_RS12675 2423491..2423841 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BKN48_RS12680 2423955..2424779 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BKN48_RS12685 2424784..2425149 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BKN48_RS12690 2425153..2425554 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
BKN48_RS12695 2425566..2426573 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
BKN48_RS12700 2426642..2426971 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
BKN48_RS12705 2426968..2427450 + 483 WP_021481702.1 anti-sigma B factor RsbW -
BKN48_RS12710 2427416..2428204 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BKN48_RS12715 2428204..2428803 + 600 WP_070548596.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T67972 WP_003156187.1 NZ_CP017676:2423491-2423841 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T67972 NZ_CP017676:2423491-2423841 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT67972 WP_003225183.1 NZ_CP017676:2423205-2423486 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT67972 NZ_CP017676:2423205-2423486 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References