Detailed information of TA system TA067968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1847752..1848329
• Replicon: chromosome
• Accession: NZ_CP017671
• Organism: Providencia rettgeri strain RB151

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RB151_RS08270
• Protein ID: WP_071547961.1
• Coordinates: 1847752..1848084 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U5N340
• Locus tag: RB151_RS08275
• Protein ID: WP_023159984.1
• Coordinates: 1848084..1848329 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RB151_RS08255	1844024..1846732	-	2709	WP_071547959.1	class I SAM-dependent DNA methyltransferase	-
RB151_RS22915	1846800..1847592	-	793	Protein_1603	IS5 family transposase	-
RB151_RS08270	1847752..1848084	-	333	WP_071547961.1	endoribonuclease MazF	Toxin
RB151_RS08275	1848084..1848329	-	246	WP_023159984.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RB151_RS08280	1848650..1849273	+	624	WP_071547962.1	AAA family ATPase	-
RB151_RS08285	1849365..1849592	+	228	WP_071547963.1	DNA partition complex ParG	-
RB151_RS08290	1849689..1850057	-	369	WP_071547964.1	hypothetical protein	-
RB151_RS08295	1850412..1850804	-	393	WP_071547965.1	hypothetical protein	-
RB151_RS08300	1850813..1851235	-	423	WP_071547966.1	hypothetical protein	-
RB151_RS08305	1851228..1851572	-	345	WP_071547967.1	single-stranded DNA-binding protein	-
RB151_RS08310	1851575..1852621	-	1047	WP_071547968.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	dfrA6	flhD / flhC / motA / cheW / cheY / flhA / flgC / flgG / flgI / fliQ / fliP / fliN / fliM / fliI / fliG / fliA	1658998..1868196	209198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T67968 WP_071547961.1 NZ_CP017671:c1848084-1847752 [Providencia rettgeri]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECEISNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T67968 NZ_CP017671:c1848084-1847752 [Providencia rettgeri]
ATGGTATCTCGCTACGTGCCTGATTCTGGTGATTTGATTTGGATTGATTTTGATCCAGTTGAGGGACATGAGCAGGGCGG
TCATCGTCCTGCTGTTGTCCTCAGTCCATTTGCATACAATAATAAAGTAGGGCTCTTACTATGCGTACCATGTACAACTA
AAGGCAAAGGCTATCCATTTGAATGCGAGATCTCGAACGAAAGAGACAGCTACGCATTAGCTGATCAAGTTACTTGTGTT
GACTGGAGAGCTCGTAAGGTTACTAAAAAAGGCAAAGTGGAAGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8786.20 Da
• Isoelectric Point: 5.0351

>AT67968 WP_023159984.1 NZ_CP017671:c1848329-1848084 [Providencia rettgeri]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT67968 NZ_CP017671:c1848329-1848084 [Providencia rettgeri]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCAATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGATAATTTGTTAGCCGGTATCACACCGGAAAATATTCATGAGAAAGTAGATTTCGGAGCTCGCACAGGTAAGGAATTA
ATATAA

Structures

Antitoxin

• AlphaFold DB: A0A857E7M9