Detailed information of TA system TA067966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 67454..68013
• Replicon: plasmid pO157
• Accession: NZ_CP017670
• Organism: Escherichia coli strain PA20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: AU473_RS28730
• Protein ID: WP_000421248.1
• Coordinates: 67738..68013 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: AU473_RS28725
• Protein ID: WP_001178089.1
• Coordinates: 67454..67738 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU473_RS28685	62646..63206	+	561	WP_000840472.1	fertility inhibition protein FinO	-
AU473_RS28690	63339..63551	+	213	WP_001302189.1	ANR family transcriptional regulator	-
AU473_RS28695	63796..64257	+	462	WP_001233853.1	thermonuclease family protein	-
AU473_RS28700	64303..64512	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
AU473_RS28705	64550..64888	+	339	WP_000766796.1	hypothetical protein	-
AU473_RS28710	65128..65382	+	255	WP_000083831.1	replication regulatory protein RepA	-
AU473_RS28715	65618..65692	+	75	WP_001370046.1	RepA leader peptide Tap	-
AU473_RS28720	65685..66542	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
AU473_RS28725	67454..67738	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
AU473_RS28730	67738..68013	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AU473_RS28735	68108..68314	+	207	WP_001105064.1	hypothetical protein	-
AU473_RS28740	68414..68629	-	216	Protein_76	transposase	-
AU473_RS31315	68673..69239	-	567	Protein_77	IS3-like element IS600 family transposase	-
AU473_RS28750	69239..69655	-	417	Protein_78	IS91 family transposase	-
AU473_RS28755	69655..69840	-	186	WP_001302179.1	hypothetical protein	-
AU473_RS28760	70017..72227	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
AU473_RS28765	72271..72660	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE	1..92755	92755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T67966 WP_000421248.1 NZ_CP017670:67738-68013 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T67966 NZ_CP017670:67738-68013 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT67966 WP_001178089.1 NZ_CP017670:67454-67738 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT67966 NZ_CP017670:67454-67738 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8