Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21817..22342 Replicon plasmid pO157
Accession NZ_CP017670
Organism Escherichia coli strain PA20

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AU473_RS28490 Protein ID WP_001159868.1
Coordinates 22037..22342 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AU473_RS28485 Protein ID WP_000813634.1
Coordinates 21817..22035 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AU473_RS31220 17100..17330 + 231 WP_010891288.1 hypothetical protein -
AU473_RS28450 17451..18191 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
AU473_RS28455 18476..19453 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
AU473_RS28465 19861..20061 - 201 WP_000708307.1 hypothetical protein -
AU473_RS28470 20058..20678 - 621 WP_001248529.1 hypothetical protein -
AU473_RS28475 20675..21358 - 684 WP_010891291.1 DNA-binding protein -
AU473_RS28485 21817..22035 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AU473_RS28490 22037..22342 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AU473_RS28495 22343..23149 + 807 WP_000016989.1 site-specific integrase -
AU473_RS31230 25047..25385 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
AU473_RS28510 25973..27139 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE 1..92755 92755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T67965 WP_001159868.1 NZ_CP017670:22037-22342 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T67965 NZ_CP017670:22037-22342 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT67965 WP_000813634.1 NZ_CP017670:21817-22035 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT67965 NZ_CP017670:21817-22035 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References