Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4652081..4652699 Replicon chromosome
Accession NZ_CP017669
Organism Escherichia coli strain PA20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AU473_RS24160 Protein ID WP_001291435.1
Coordinates 4652481..4652699 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AU473_RS24155 Protein ID WP_000344800.1
Coordinates 4652081..4652455 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AU473_RS24145 4647170..4648363 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AU473_RS24150 4648386..4651535 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
AU473_RS24155 4652081..4652455 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AU473_RS24160 4652481..4652699 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AU473_RS24165 4652871..4653422 + 552 WP_000102553.1 maltose O-acetyltransferase -
AU473_RS24170 4653538..4654008 + 471 WP_000136192.1 YlaC family protein -
AU473_RS24175 4654172..4655722 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AU473_RS24180 4655764..4656117 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AU473_RS24190 4656496..4656807 + 312 WP_000409911.1 MGMT family protein -
AU473_RS24195 4656840..4657412 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T67957 WP_001291435.1 NZ_CP017669:4652481-4652699 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T67957 NZ_CP017669:4652481-4652699 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT67957 WP_000344800.1 NZ_CP017669:4652081-4652455 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT67957 NZ_CP017669:4652081-4652455 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References