Detailed information of TA system TA067946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3568894..3569365
• Replicon: chromosome
• Accession: NZ_CP017669
• Organism: Escherichia coli strain PA20

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: AU473_RS18705
• Protein ID: WP_001303511.1
• Coordinates: 3569087..3569365 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: AU473_RS18700
• Protein ID: WP_001302048.1
• Coordinates: 3568894..3569085 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU473_RS18655	3564101..3564685	-	585	WP_000206793.1	DUF551 domain-containing protein	-
AU473_RS18660	3564742..3565137	-	396	WP_001118159.1	DUF977 family protein	-
AU473_RS18665	3565153..3565922	-	770	Protein_3537	DUF1627 domain-containing protein	-
AU473_RS18675	3565948..3566688	-	741	WP_000788938.1	ATP-binding protein	-
AU473_RS18680	3566695..3567657	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
AU473_RS18685	3567680..3568105	-	426	WP_000693943.1	Rha family transcriptional regulator	-
AU473_RS18690	3568102..3568404	-	303	WP_000172738.1	transcriptional regulator	-
AU473_RS30520	3568502..3568873	+	372	WP_001169686.1	hypothetical protein	-
AU473_RS18700	3568894..3569085	+	192	WP_001302048.1	hypothetical protein	Antitoxin
AU473_RS18705	3569087..3569365	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AU473_RS18715	3569718..3570017	+	300	WP_001240334.1	hypothetical protein	-
AU473_RS18720	3570089..3570307	+	219	WP_001171930.1	hypothetical protein	-
AU473_RS18725	3570876..3571064	+	189	WP_000449192.1	cell division inhibition protein DicB	-
AU473_RS18730	3571061..3571252	+	192	WP_001090200.1	DUF1482 family protein	-
AU473_RS18735	3571345..3573816	+	2472	WP_000048458.1	exonuclease	-
AU473_RS18740	3573889..3574140	+	252	WP_000005552.1	excisionase family protein	-
AU473_RS32010	3574175..3574362	+	188	Protein_3551	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3531589..3584245	52656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T67946 WP_001303511.1 NZ_CP017669:3569087-3569365 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T67946 NZ_CP017669:3569087-3569365 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT67946 WP_001302048.1 NZ_CP017669:3568894-3569085 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT67946 NZ_CP017669:3568894-3569085 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0