Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3309864..3310502 Replicon chromosome
Accession NZ_CP017669
Organism Escherichia coli strain PA20

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AU473_RS17275 Protein ID WP_000813794.1
Coordinates 3310326..3310502 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AU473_RS17270 Protein ID WP_001270286.1
Coordinates 3309864..3310280 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AU473_RS17250 3305016..3305957 - 942 WP_001251320.1 ABC transporter permease -
AU473_RS17255 3305958..3306971 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
AU473_RS17260 3306989..3308134 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
AU473_RS17265 3308379..3309785 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
AU473_RS17270 3309864..3310280 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AU473_RS17275 3310326..3310502 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AU473_RS17280 3310724..3310954 + 231 WP_000494244.1 YncJ family protein -
AU473_RS17285 3311046..3313007 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AU473_RS17290 3313080..3313616 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
AU473_RS17295 3313669..3314880 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T67945 WP_000813794.1 NZ_CP017669:c3310502-3310326 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T67945 NZ_CP017669:c3310502-3310326 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT67945 WP_001270286.1 NZ_CP017669:c3310280-3309864 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT67945 NZ_CP017669:c3310280-3309864 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References