Detailed information of TA system TA067913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 531583..532172
• Replicon: chromosome
• Accession: NZ_CP017662
• Organism: Cronobacter sp. JZ38

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: BJP61_RS02435
• Protein ID: WP_161581900.1
• Coordinates: 531804..532172 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: K8BAC3
• Locus tag: BJP61_RS02430
• Protein ID: WP_007730583.1
• Coordinates: 531583..531804 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJP61_RS02400	526633..527025	-	393	WP_038866997.1	aspartate 1-decarboxylase autocleavage activator PanM	-
BJP61_RS02405	527446..527703	+	258	WP_171997782.1	amino acid-binding protein	-
BJP61_RS02410	527768..528694	+	927	WP_038866950.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
BJP61_RS02415	528691..529971	+	1281	WP_075193300.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
BJP61_RS02420	529968..530735	+	768	WP_038866954.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
BJP61_RS02425	530737..531450	+	714	WP_038866956.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
BJP61_RS02430	531583..531804	+	222	WP_007730583.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BJP61_RS02435	531804..532172	+	369	WP_161581900.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BJP61_RS02440	532343..533662	+	1320	WP_075193298.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
BJP61_RS02445	533880..534767	+	888	WP_038866963.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
BJP61_RS02450	534764..535609	+	846	WP_038866965.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
BJP61_RS02455	535611..536684	+	1074	WP_038866967.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	529968..537421	7453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13547.86 Da
• Isoelectric Point: 6.7259

>T67913 WP_161581900.1 NZ_CP017662:531804-532172 [Cronobacter sp. JZ38]
MTIHFISAEEIVRFHDRLLQVTPGVTGMPDPGRVEALMYRVLNKYEYEGVDDIWLLAAMHLLAIARGQIFNDGNKRTALF
ITLLLLKRNGIALAANPAFVEMTVNAAAGRLTLEEIAAQLRA

Nucleotide

• Length: 369 bp

>T67913 NZ_CP017662:531804-532172 [Cronobacter sp. JZ38]
ATGACGATTCACTTTATCTCAGCGGAGGAGATAGTTCGCTTCCATGACCGCTTATTGCAGGTGACGCCCGGCGTCACAGG
GATGCCCGATCCGGGGCGGGTCGAAGCGTTAATGTATCGCGTGCTTAATAAATATGAGTACGAAGGCGTTGACGATATCT
GGCTGCTGGCGGCGATGCATTTACTGGCGATTGCGCGAGGCCAAATTTTCAACGACGGTAATAAGCGCACGGCGCTTTTC
ATTACGCTGTTGCTTCTTAAGCGAAACGGTATCGCGCTTGCGGCTAATCCGGCTTTTGTTGAGATGACCGTCAACGCGGC
GGCAGGGCGTCTGACCCTGGAAGAGATAGCTGCCCAGCTTCGCGCTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8168.20 Da
• Isoelectric Point: 5.6746

>AT67913 WP_007730583.1 NZ_CP017662:531583-531804 [Cronobacter sp. JZ38]
MRTMNYSEARQQLASALESATTGTPVTITRRGHKPAVIISAEEFERYQAAKLDAEFAEMMAVHGNEIRELADR

Nucleotide

• Length: 222 bp

>AT67913 NZ_CP017662:531583-531804 [Cronobacter sp. JZ38]
ATGCGTACGATGAACTACAGCGAAGCGCGCCAGCAGCTCGCTTCCGCCCTGGAATCCGCCACCACCGGAACGCCAGTGAC
CATTACGCGCCGCGGCCATAAACCGGCCGTTATCATCAGCGCCGAAGAGTTTGAACGTTACCAGGCGGCCAAACTGGATG
CCGAATTCGCGGAGATGATGGCCGTGCACGGTAATGAAATCAGGGAGCTTGCGGACAGGTAA

Structures

Antitoxin

• AlphaFold DB: K8BAC3