Detailed information of TA system TA067874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1214560..1215313
• Replicon: chromosome
• Accession: NZ_CP017638
• Organism: Dickeya dianthicola RNS04.9

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A3A4D4W2
• Locus tag: DDI_RS05855
• Protein ID: WP_024105250.1
• Coordinates: 1214560..1214817 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: DDI_RS05860
• Protein ID: WP_024105251.1
• Coordinates: 1214825..1215313 (+)
• Length: 163 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDI_RS05840	1209990..1210991	-	1002	WP_026594706.1	putative DNA binding domain-containing protein	-
DDI_RS05845	1211327..1213477	-	2151	WP_024105249.1	formate dehydrogenase subunit alpha	-
DDI_RS05850	1213512..1214054	-	543	WP_013317225.1	4Fe-4S dicluster domain-containing protein	-
DDI_RS05855	1214560..1214817	+	258	WP_024105250.1	DUF4160 domain-containing protein	Toxin
DDI_RS05860	1214825..1215313	+	489	WP_024105251.1	addiction module antitoxin	Antitoxin
DDI_RS05865	1215432..1215929	-	498	WP_024105252.1	hydrogenase maturation peptidase HycI	-
DDI_RS05870	1215922..1216320	-	399	WP_024105253.1	formate hydrogenlyase maturation HycH family protein	-
DDI_RS05875	1216317..1217108	-	792	WP_024105254.1	NADH-quinone oxidoreductase subunit B family protein	-
DDI_RS05880	1217105..1217674	-	570	WP_024105255.1	hydrogenase 4 subunit H	-
DDI_RS05885	1217685..1219412	-	1728	WP_024105256.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (80-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10039.68 Da
• Isoelectric Point: 8.9654

>T67874 WP_024105250.1 NZ_CP017638:1214560-1214817 [Dickeya dianthicola RNS04.9]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGHHELIISIRTGIEIEGYLPNNKRKLALVHIRAHRQQLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T67874 NZ_CP017638:1214560-1214817 [Dickeya dianthicola RNS04.9]
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATGATGAACGGCACCACTTGCCGCATGT
GCATGTTTGTTATGGCCATCATGAACTTATCATCAGTATTCGTACCGGAATCGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGCTGGCGCTTGTGCATATCCGGGCCCACCGCCAACAGCTGTTGAGCATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCGGGAAAACTGTGA

Antitoxin

Protein

• Length: 163 a.a.
• Molecular weight: 18054.66 Da
• Isoelectric Point: 6.9793

>AT67874 WP_024105251.1 NZ_CP017638:1214825-1215313 [Dickeya dianthicola RNS04.9]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYHYAPSPVFSNASQFIKFGLLPDGSLEWLPDIKISASELRKKGEYVSHTLA
VRERNFADVISRAFYDAVIENRPEILQAALKIAVEKYGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Nucleotide

• Length: 489 bp

>AT67874 NZ_CP017638:1214825-1215313 [Dickeya dianthicola RNS04.9]
ATGCATAAAATTGTGGAAGTTGACGTCATTGGCGATTTTTCGTTGCGCCTGACCTATGAAGATGGTCTGGTTTGCGACAT
CAATCTTTATCACTATGCCCCCAGCCCGGTATTTTCGAACGCCAGCCAATTTATCAAGTTTGGGTTGCTGCCGGATGGCT
CGCTGGAATGGCTGCCGGATATTAAGATTTCCGCGTCTGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTGGCG
GTGCGAGAACGGAACTTCGCTGATGTCATCTCTCGCGCTTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATTGCGGTGGAGAAATACGGTAACAGCACGGTGGCGAAAGAGGCGGGGGCGAAAAGCCGCACCAGTC
TGTATAAGTCGCTGAATAAGAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAACTGGAG
GAGAAGTAG

Structures

Toxin

• AlphaFold DB: A0A3A4D4W2