Detailed information of TA system TA067873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 954431..955259
• Replicon: chromosome
• Accession: NZ_CP017638
• Organism: Dickeya dianthicola RNS04.9

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A3A4CQM7
• Locus tag: DDI_RS04600
• Protein ID: WP_024105035.1
• Coordinates: 954431..954967 (-)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: DDI_RS04605
• Protein ID: WP_024105036.1
• Coordinates: 954948..955259 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDI_RS04580	949796..950764	+	969	WP_024105031.1	o-succinylbenzoate synthase	-
DDI_RS04585	950752..952122	+	1371	WP_024105032.1	o-succinylbenzoate--CoA ligase	-
DDI_RS04590	952329..952874	+	546	WP_024105033.1	membrane protein	-
DDI_RS04595	953077..954270	+	1194	WP_024105034.1	nicotinamide mononucleotide deamidase-related protein YfaY	-
DDI_RS04600	954431..954967	-	537	WP_024105035.1	GNAT family N-acetyltransferase	Toxin
DDI_RS04605	954948..955259	-	312	WP_024105036.1	DUF1778 domain-containing protein	Antitoxin
DDI_RS04610	955558..955853	-	296	Protein_886	IS4 family transposase	-
DDI_RS04615	955956..956816	-	861	WP_024105037.1	co-chaperone YbbN	-
DDI_RS04620	956879..957655	-	777	WP_024105038.1	SDR family oxidoreductase	-
DDI_RS04625	957801..958442	-	642	WP_024105039.1	multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1	-
DDI_RS04630	958410..959096	+	687	WP_024107942.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-154)

Antitoxin

• Vibrio phage ICP1, Orf50 (18-96)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19327.27 Da
• Isoelectric Point: 6.7055

>T67873 WP_024105035.1 NZ_CP017638:c954967-954431 [Dickeya dianthicola RNS04.9]
MVDTQSESNGVVVYAYQADTTYPGSKSFDCGNTVINSFVRSSLKKSVRDGNCAAKVLVKDETKELLGFCTFAAYSLEKSK
LAGVVSGSLPHELGVVRLIMLGVATKEQNKGYGQELLLEFFKQVKTIHESLPVKGVYLDADPAAIDFYIRLGFVQLNEPP
NAFGAVPMFLAIQHILAA

Nucleotide

• Length: 537 bp

>T67873 NZ_CP017638:c954967-954431 [Dickeya dianthicola RNS04.9]
ATGGTAGATACTCAAAGTGAAAGTAATGGTGTAGTGGTATACGCGTATCAGGCAGATACCACATACCCTGGGAGTAAGAG
TTTCGATTGCGGAAATACCGTAATCAACTCTTTTGTGCGTAGCTCCCTCAAGAAAAGCGTCAGAGATGGGAATTGTGCAG
CTAAAGTATTGGTTAAAGATGAAACAAAAGAGCTGTTGGGTTTCTGTACTTTTGCTGCTTACTCTCTTGAAAAAAGTAAG
TTGGCGGGGGTGGTTTCAGGTTCATTGCCTCACGAATTAGGGGTTGTTCGGCTAATTATGCTGGGGGTAGCCACGAAAGA
ACAGAATAAAGGCTATGGACAAGAACTGCTTTTAGAATTCTTTAAACAGGTTAAAACCATCCACGAATCGCTACCTGTTA
AAGGCGTGTATTTGGATGCGGATCCGGCAGCAATTGACTTCTATATCCGGTTAGGTTTCGTTCAACTTAATGAACCACCG
AATGCTTTTGGTGCGGTGCCCATGTTTTTGGCCATTCAACATATCCTTGCTGCGTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11448.09 Da
• Isoelectric Point: 10.7931

>AT67873 WP_024105036.1 NZ_CP017638:c955259-954948 [Dickeya dianthicola RNS04.9]
MSNVSVFPNDIRVTKNARVELKTSPDLKDVLREAATAAGLDLSAFILNAALERAESVLDNQRRRQLSAASWQQLNQLLSE
PAAPTLALQALMRRKKQDGRYSK

Nucleotide

• Length: 312 bp

>AT67873 NZ_CP017638:c955259-954948 [Dickeya dianthicola RNS04.9]
ATGTCTAATGTATCAGTGTTTCCCAATGATATTCGCGTCACGAAGAACGCGCGGGTGGAGTTAAAAACTAGCCCTGACCT
TAAAGATGTGCTTCGTGAAGCAGCTACGGCTGCAGGTTTAGATTTGAGTGCGTTTATTCTTAATGCCGCCCTGGAGCGAG
CAGAAAGCGTCCTGGATAACCAGCGGCGCCGACAGCTTTCAGCAGCAAGCTGGCAACAATTAAACCAACTCTTGTCTGAA
CCTGCTGCACCGACGCTGGCTTTACAGGCGTTAATGCGGAGGAAGAAACAAGATGGTAGATACTCAAAGTGA

Structures

Toxin

• AlphaFold DB: A0A3A4CQM7