Detailed information of TA system TA067871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 698117..699837
• Replicon: chromosome
• Accession: NZ_CP017638
• Organism: Dickeya dianthicola RNS04.9

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: DDI_RS03445
• Protein ID: WP_024104823.1
• Coordinates: 699181..699837 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A3A4C958
• Locus tag: DDI_RS03440
• Protein ID: WP_024104822.1
• Coordinates: 698117..699184 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDI_RS03415	693257..693583	-	327	Protein_659	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
DDI_RS21865	693575..693703	-	129	Protein_660	DUF4102 domain-containing protein	-
DDI_RS03420	693811..693993	-	183	WP_024109619.1	hypothetical protein	-
DDI_RS03425	694142..694858	-	717	WP_024104820.1	M48 family metallopeptidase	-
DDI_RS03430	694858..697953	-	3096	WP_024104821.1	HsdR family type I site-specific deoxyribonuclease	-
DDI_RS03440	698117..699184	-	1068	WP_024104822.1	macro domain-containing protein	Antitoxin
DDI_RS03445	699181..699837	-	657	WP_024104823.1	DUF4433 domain-containing protein	Toxin
DDI_RS03450	699840..700415	-	576	WP_024104824.1	DUF4433 domain-containing protein	-
DDI_RS03455	700420..701769	-	1350	WP_051119513.1	restriction endonuclease subunit S	-
DDI_RS03460	701781..704216	-	2436	WP_024104826.1	type I restriction-modification system subunit M	-
DDI_RS03465	704256..704495	-	240	WP_024104827.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	677192..706203	29011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25142.59 Da
• Isoelectric Point: 7.0165

>T67871 WP_024104823.1 NZ_CP017638:c699837-699181 [Dickeya dianthicola RNS04.9]
VAYDYSANLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAANWINIGNPELIDKRAGHPVTAGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSSLRHVAIQNVPFVFTDSHAYYNLANYYTDLNNLHRIDWPILQARDFRRDQDD
LAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T67871 NZ_CP017638:c699837-699181 [Dickeya dianthicola RNS04.9]
GTGGCCTATGACTACAGCGCCAATCTCAACCCGGAAAAGGCCCTTATCTGGCGCATTGTCCATCGGGACAATATCCCGTG
GATACTGGATAACGGCCTGCACTGCGGCAACAGCCCGGTGCAGGCCGCAAACTGGATCAATATCGGCAACCCGGAGCTTA
TCGACAAACGCGCCGGGCATCCGGTGACTGCCGGGACGGGTGGGACGCTGCATGACTATGTGCCGTTTTACTTTACCCCC
TTCTCACCGATGCTGATGAATATCCACAGTGGGCGCGGCGGCATTAAGCAACGTCCCAACGAAGAAATCGTCATTCTGGT
CAGCAGCCTGCGACATGTGGCGATACAAAACGTCCCCTTCGTGTTTACCGACAGCCACGCCTACTATAATCTGGCGAATT
ACTATACCGATCTAAACAATCTGCACCGGATCGACTGGCCCATTTTACAGGCGCGGGATTTCCGTCGCGATCAGGACGAT
CTTGCCAAGTTTGAACGCTATCAGGCCGAGGCGTTAATCTGGCAGCACTGCCCCATCACGCTGCTGGTCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAATGGCTGTCGCAACGCAATCTGACAATGCCCGTTCACGCACGGGCCG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40767.89 Da
• Isoelectric Point: 5.4873

>AT67871 WP_024104822.1 NZ_CP017638:c699184-698117 [Dickeya dianthicola RNS04.9]
MMTYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYALACKQKQVTTGKMFITETDELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWSDVQARIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHNYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNNEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGECQPTLDSVKEG
LHQWPAGERWASRKMKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT67871 NZ_CP017638:c699184-698117 [Dickeya dianthicola RNS04.9]
ATGATGACATATACACAAGGCAATTTACTGGACGCGCCCGTGGAAGCACTGGTGAATACGGTGAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTCAAAGAGCGCTTCCCCGATAATATGAAAGCCTATGCACTCGCCTGTAAACAAAAGC
AGGTGACGACTGGCAAAATGTTTATCACTGAAACCGACGAGCTGATGGGGCCGCGCTGGATTGTCAACTTCCCCACCAAG
CAGCACTGGCGCGCCGACTCTCGCATGGAGTGGATTGAAGACGGCTTACAGGACTTACGTCGCTTTTTGATCGAAGAGAA
AGTGCAGTCCATCGCCATTCCTCCGCTGGGGGCGGGAAACGGCGGCCTGAACTGGTCGGACGTGCAGGCGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATCCTGATTTATGAGCCTACCGAAAAATACCAAAACGTCGCCAAGAGTACC
GGCGTAAAAAAGCTCACCCCCGCCAGAGCGATGATCGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGTTACAACGCGCCATTGAACAGCACCAGCAGGAGGACGTGCTAAAACTGC
GGTTTGAGGCACACAATTATGGCCCGTATGCCCCCAACCTGAACCATCTGCTTAACGCGCTGGACGGCACCTATCTGAAG
GCCGAGAAACGCATCCCGGACAGCCAGCCGCTGGACGTGATCTGGTTTAACGATCGGGAAAAAGAACACATTAATCTCTG
GCTGAATAACGAGGCCAAAGCGTGGCTACCGGCGCTTGAGCAGGTCAGCCAGTTGATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGCTGCTGGCGACCGTTGACTGGCTGCTGACCCGAGGAGAGTGCCAGCCCACGCTGGATTCCGTGAAAGAGGGG
CTACACCAGTGGCCTGCCGGTGAACGCTGGGCCAGCCGTAAGATGAAGCTCTTTGACGACAAAAATCTGCAATTTGCCAT
AAACCGTGTCATGGAGTTCCATTGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3A4C958