Detailed information of TA system TA067868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 186487..187229
• Replicon: chromosome
• Accession: NZ_CP017638
• Organism: Dickeya dianthicola RNS04.9

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: E0SIC1
• Locus tag: DDI_RS00895
• Protein ID: WP_013316117.1
• Coordinates: 186487..186966 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3A4C8A5
• Locus tag: DDI_RS00900
• Protein ID: WP_024104363.1
• Coordinates: 186957..187229 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDI_RS00860	181730..182053	+	324	WP_024104357.1	DNA-binding transcriptional regulator	-
DDI_RS00865	182349..183611	+	1263	WP_024104358.1	AAA family ATPase	-
DDI_RS00870	183611..184240	+	630	WP_024104359.1	DUF4276 family protein	-
DDI_RS00875	185165..185485	+	321	WP_024104360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DDI_RS00880	185475..185759	+	285	WP_024104361.1	helix-turn-helix domain-containing protein	-
DDI_RS00890	186057..186458	-	402	WP_024104362.1	hypothetical protein	-
DDI_RS00895	186487..186966	-	480	WP_013316117.1	GNAT family N-acetyltransferase	Toxin
DDI_RS00900	186957..187229	-	273	WP_024104363.1	DUF1778 domain-containing protein	Antitoxin
DDI_RS00905	187719..188142	+	424	Protein_176	DUF4102 domain-containing protein	-
DDI_RS00910	188154..188998	-	845	Protein_177	IS5 family transposase	-
DDI_RS00915	189050..189271	-	222	WP_024104365.1	DUF1778 domain-containing protein	-
DDI_RS21980	189342..189410	+	69	Protein_179	integrase	-
DDI_RS00930	189569..190957	+	1389	WP_024104366.1	hypothetical protein	-
DDI_RS00935	191107..191445	-	339	WP_024104367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	174133..197106	22973
	-	flank	IS/Tn	-	-	188573..188887	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17564.34 Da
• Isoelectric Point: 9.5367

>T67868 WP_013316117.1 NZ_CP017638:c186966-186487 [Dickeya dianthicola RNS04.9]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVPSEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T67868 NZ_CP017638:c186966-186487 [Dickeya dianthicola RNS04.9]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10390.84 Da
• Isoelectric Point: 4.6702

>AT67868 WP_024104363.1 NZ_CP017638:c187229-186957 [Dickeya dianthicola RNS04.9]
MRTETKETPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRMN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT67868 NZ_CP017638:c187229-186957 [Dickeya dianthicola RNS04.9]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A3N0G158

Antitoxin

• AlphaFold DB: A0A3A4C8A5