Detailed information of TA system TA067867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8171261..8171832
• Replicon: chromosome
• Accession: NZ_CP017637
• Organism: Bradyrhizobium japonicum strain J5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1L3FM63
• Locus tag: BKD09_RS38925
• Protein ID: WP_063985200.1
• Coordinates: 8171479..8171832 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8T5V1Z1
• Locus tag: BKD09_RS38920
• Protein ID: WP_063985199.1
• Coordinates: 8171261..8171482 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKD09_RS38910	8166661..8169798	-	3138	WP_071916223.1	efflux RND transporter permease subunit	-
BKD09_RS38915	8169975..8171081	-	1107	WP_028148596.1	efflux RND transporter periplasmic adaptor subunit	-
BKD09_RS38920	8171261..8171482	+	222	WP_063985199.1	antitoxin MazE family protein	Antitoxin
BKD09_RS38925	8171479..8171832	+	354	WP_063985200.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BKD09_RS38930	8171934..8173265	-	1332	WP_071916224.1	MFS transporter	-
BKD09_RS38935	8173334..8173774	+	441	WP_155795385.1	DUF2809 domain-containing protein	-
BKD09_RS38940	8173898..8175061	-	1164	WP_071916226.1	winged helix-turn-helix domain-containing protein	-
BKD09_RS38945	8175294..8175539	-	246	WP_071916227.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (11-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12602.39 Da
• Isoelectric Point: 6.3275

>T67867 WP_063985200.1 NZ_CP017637:8171479-8171832 [Bradyrhizobium japonicum]
MKRGEVWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDGNGLRAPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAILVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T67867 NZ_CP017637:8171479-8171832 [Bradyrhizobium japonicum]
ATGAAGCGAGGTGAGGTCTGGACGGTGGCAGGCGGCAAGGACTATGCGGGCAAGCCGCGCCCCGTCGTCATCGTGCAGGA
CGACAGCTTTGACGCGACGAACTCCATCACTGTCTGCGCCTTCACGACCGACGAAACCGAGGCGCCACTTTTCCGGCTCA
AGGTCGAGCCGAACGACGGCAACGGCCTTCGAGCTCCGTCTCGACTTATGGTCGACAAGATTACTACAGTGCCAAAAGCC
AAGGTAGGAACGTTAGTGGGACGCCTAGACGATGACGACCTGCTGCGTCTCAATCGCGCTATTCTGGTGTTTCTCGGACT
GGCTGGTTCGCAGAGAAGCGCCAGTGCAGATTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8284.21 Da
• Isoelectric Point: 7.3202

>AT67867 WP_063985199.1 NZ_CP017637:8171261-8171482 [Bradyrhizobium japonicum]
MSPSKPKPSRMKVQAHRDRLRAQGLRPIQIWVPDIRSPSFQSEAHRQSLAVATSSHESEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT67867 NZ_CP017637:8171261-8171482 [Bradyrhizobium japonicum]
ATGAGCCCTTCAAAGCCCAAGCCGTCTCGTATGAAGGTGCAGGCCCACCGTGATCGATTGCGTGCACAAGGCCTGCGGCC
GATCCAGATCTGGGTGCCGGATATCCGCTCGCCCTCCTTCCAATCGGAAGCCCATCGCCAATCGCTTGCGGTCGCAACGA
GCTCACATGAATCCGAAGACCAAGGCTTCATTGATGCCGTATCCGATTGGACCGATGAATGA

Structures

Toxin

• AlphaFold DB: A0A1L3FM63