Detailed information of TA system TA067859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 712736..713252
• Replicon: chromosome
• Accession: NZ_CP017635
• Organism: Vibrio vulnificus Env1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: BJD94_RS03845
• Protein ID: WP_005464459.1
• Coordinates: 712983..713252 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7W4CB60
• Locus tag: BJD94_RS03840
• Protein ID: WP_005483180.1
• Coordinates: 712736..712990 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD94_RS03800	707955..708872	+	918	WP_103154878.1	hypothetical protein	-
BJD94_RS03810	709338..709460	+	123	WP_088900138.1	DUF3265 domain-containing protein	-
BJD94_RS03815	709495..709890	+	396	WP_000870862.1	DUF3465 domain-containing protein	-
BJD94_RS23475	710050..710535	+	486	WP_193783804.1	hypothetical protein	-
BJD94_RS03820	710678..710953	+	276	WP_103154884.1	antibiotic biosynthesis monooxygenase	-
BJD94_RS03825	710987..711955	-	969	WP_103154883.1	IS30 family transposase	-
BJD94_RS23480	712573..712644	+	72	WP_114983889.1	hypothetical protein	-
BJD94_RS03840	712736..712990	+	255	WP_005483180.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BJD94_RS03845	712983..713252	+	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
BJD94_RS03855	713836..713963	+	128	Protein_729	DUF3265 domain-containing protein	-
BJD94_RS23390	713956..714288	+	333	WP_159074658.1	hypothetical protein	-
BJD94_RS03865	715043..715714	+	672	WP_103154881.1	hypothetical protein	-
BJD94_RS23485	715732..715821	+	90	WP_083389094.1	DUF3265 domain-containing protein	-
BJD94_RS03875	716162..716287	+	126	WP_081946464.1	DUF3265 domain-containing protein	-
BJD94_RS03885	716648..716776	+	129	WP_080540726.1	DUF3265 domain-containing protein	-
BJD94_RS03895	717264..717593	+	330	WP_026050567.1	hypothetical protein	-
BJD94_RS03900	717566..717727	+	162	WP_103155364.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flp1	361742..900507	538765
	-	flank	IS/Tn	-	-	710987..711955	968
	-	inside	Integron	-	-	690251..800877	110626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T67859 WP_005464459.1 NZ_CP017635:712983-713252 [Vibrio vulnificus Env1]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T67859 NZ_CP017635:712983-713252 [Vibrio vulnificus Env1]
ATGAGTAGTAGTCAGCGTTTACTGTCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAAAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGTTCTCCATTTGAGGGCATCGGTAAACCTGAGCCATTGA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAGGCCATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9481.66 Da
• Isoelectric Point: 5.0623

>AT67859 WP_005483180.1 NZ_CP017635:712736-712990 [Vibrio vulnificus Env1]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT67859 NZ_CP017635:712736-712990 [Vibrio vulnificus Env1]
ATGAGAATCGTATCTTTTACTGAAGCAAGAAATGGTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGTCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACAGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGTGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8

Antitoxin

• AlphaFold DB: A0A7W4CB60