Detailed information of TA system TA067856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 2834143..2834768
• Replicon: chromosome
• Accession: NZ_CP017634
• Organism: Candidatus Formimonas warabiya strain DCMF

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DCMF_RS13125
• Protein ID: WP_148134828.1
• Coordinates: 2834143..2834493 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DCMF_RS13130
• Protein ID: WP_214659321.1
• Coordinates: 2834490..2834768 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCMF_RS13095 (DCMF_13100)	2829436..2829912	-	477	WP_148134824.1	ribosomal protein S18-alanine N-acetyltransferase	-
DCMF_RS13100 (DCMF_13105)	2829909..2830622	-	714	WP_148134825.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
DCMF_RS13105 (DCMF_13110)	2830603..2831079	-	477	WP_236860330.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
DCMF_RS13110 (DCMF_13115)	2831156..2831713	-	558	WP_148137897.1	uracil-DNA glycosylase	-
DCMF_RS13115 (DCMF_13120)	2831994..2833103	-	1110	WP_148137898.1	amidohydrolase	-
DCMF_RS13120 (DCMF_13125)	2833370..2834083	-	714	WP_148134827.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DCMF_RS13125 (DCMF_13130)	2834143..2834493	-	351	WP_148134828.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DCMF_RS13130 (DCMF_13135)	2834490..2834768	-	279	WP_214659321.1	ribbon-helix-helix protein, CopG family	Antitoxin
DCMF_RS13135 (DCMF_13140)	2834850..2836016	-	1167	WP_148134830.1	alanine racemase	-
DCMF_RS13140 (DCMF_13145)	2836013..2837569	-	1557	WP_148134831.1	NAD(P)H-hydrate dehydratase	-
DCMF_RS13145 (DCMF_13150)	2837517..2837951	-	435	WP_148134832.1	holo-ACP synthase	-
DCMF_RS13150 (DCMF_13155)	2838181..2838504	-	324	WP_148134833.1	hypothetical protein	-
DCMF_RS30095	2838528..2838653	-	126	WP_257790960.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12816.82 Da
• Isoelectric Point: 6.4740

>T67856 WP_148134828.1 NZ_CP017634:c2834493-2834143 [Candidatus Formimonas warabiya]
MIVRRGEVFFAELNPVQGSEQGGTRPVLVVQNDVGNQYSPTTIVVAITSQINKAKLPTHVELPKEKTGLDKDSVILAEQV
RTIDKSRLQHKVAMLGEEIMNRVDRALEISLGLVEI

Nucleotide

• Length: 351 bp

>T67856 NZ_CP017634:c2834493-2834143 [Candidatus Formimonas warabiya]
ATGATTGTACGGCGCGGGGAAGTGTTTTTTGCCGAGCTAAATCCTGTCCAAGGTTCAGAGCAGGGAGGCACCAGGCCTGT
TTTGGTAGTACAAAATGATGTCGGCAACCAATACAGCCCAACTACTATTGTTGTTGCCATTACCTCTCAGATTAACAAAG
CAAAATTGCCTACGCATGTAGAACTGCCTAAAGAAAAAACAGGGCTTGATAAAGATTCGGTAATTTTAGCCGAACAGGTA
CGCACCATTGACAAAAGCCGCCTTCAGCATAAGGTTGCCATGCTGGGGGAAGAGATTATGAATCGGGTAGACCGGGCATT
GGAGATCAGTTTAGGATTAGTGGAAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 11010.68 Da
• Isoelectric Point: 7.2455

>AT67856 WP_214659321.1 NZ_CP017634:c2834768-2834490 [Candidatus Formimonas warabiya]
VPNLKRIMISIPDQLLQEFDGLVNHENKNRSEFIREAMKFYIAERRRRILHEQMKKGYQEMANINLSIARECFSMEHEAF
EDPGEKIAECRK

Nucleotide

• Length: 279 bp

>AT67856 NZ_CP017634:c2834768-2834490 [Candidatus Formimonas warabiya]
GTGCCGAATTTAAAGCGAATTATGATTAGTATCCCGGATCAGCTGCTGCAAGAGTTTGATGGATTGGTGAATCATGAAAA
TAAGAACAGGAGCGAGTTTATCCGGGAAGCGATGAAGTTTTATATTGCCGAACGTCGCCGGCGCATCCTGCATGAACAGA
TGAAAAAGGGATATCAGGAGATGGCAAATATTAATTTATCTATTGCCCGGGAATGTTTCAGTATGGAACATGAGGCCTTT
GAAGACCCCGGAGAAAAAATAGCGGAGTGTCGCAAATGA