Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 22457..22979 Replicon plasmid pSLK172-2
Accession NZ_CP017633
Organism Escherichia coli strain SLK172

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag BJJ90_RS31040 Protein ID WP_000220561.1
Coordinates 22457..22738 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BJJ90_RS31045 Protein ID WP_000121743.1
Coordinates 22728..22979 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJJ90_RS31015 17933..18169 - 237 Protein_18 hypothetical protein -
BJJ90_RS31020 18190..18984 - 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
BJJ90_RS31025 19013..20443 - 1431 Protein_20 IS4-like element IS50R family transposase -
BJJ90_RS31030 20563..20751 + 189 WP_000957857.1 hypothetical protein -
BJJ90_RS31035 20761..21960 + 1200 WP_072648941.1 IS91 family transposase -
BJJ90_RS33570 22126..22218 + 93 Protein_23 IS6 family transposase -
BJJ90_RS31040 22457..22738 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BJJ90_RS31045 22728..22979 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BJJ90_RS31060 24214..25053 + 840 WP_024129959.1 replication initiation protein -
BJJ90_RS31065 25074..25916 + 843 WP_000435064.1 replication initiation protein -
BJJ90_RS31070 25920..26366 + 447 WP_001074384.1 hypothetical protein -
BJJ90_RS31075 26434..26856 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR - 1..120528 120528


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T67846 WP_000220561.1 NZ_CP017633:c22738-22457 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T67846 NZ_CP017633:c22738-22457 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT67846 WP_000121743.1 NZ_CP017633:c22979-22728 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT67846 NZ_CP017633:c22979-22728 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References