Detailed information of TA system TA067844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 123220..123821
• Replicon: plasmid pSLK172-1
• Accession: NZ_CP017632
• Organism: Escherichia coli strain SLK172

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: BJJ90_RS29405
• Protein ID: WP_001216034.1
• Coordinates: 123441..123821 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BJJ90_RS29400
• Protein ID: WP_001190712.1
• Coordinates: 123220..123441 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJJ90_RS29355	118807..119232	+	426	WP_069723429.1	ead/Ea22-like family protein	-
BJJ90_RS33545	119234..119412	+	179	Protein_121	hypothetical protein	-
BJJ90_RS29365	119746..120732	+	987	WP_069723428.1	hypothetical protein	-
BJJ90_RS29370	120729..121091	+	363	WP_001261544.1	hypothetical protein	-
BJJ90_RS33710	121166..121303	-	138	WP_000123562.1	hypothetical protein	-
BJJ90_RS29385	121753..122004	+	252	WP_069723427.1	DNA polymerase III subunit theta	-
BJJ90_RS29390	122011..122583	-	573	WP_069723426.1	DNA-binding protein	-
BJJ90_RS29395	122758..123147	+	390	WP_000506730.1	DNA repair protein	-
BJJ90_RS29400	123220..123441	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BJJ90_RS29405	123441..123821	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BJJ90_RS29410	123826..124005	+	180	WP_001339207.1	hypothetical protein	-
BJJ90_RS29415	124033..125076	+	1044	WP_069723425.1	DUF968 domain-containing protein	-
BJJ90_RS29420	125165..125617	+	453	WP_001326849.1	late promoter-activating protein	-
BJJ90_RS29425	125703..126896	+	1194	WP_069723424.1	terminase	-
BJJ90_RS29430	126896..128380	+	1485	WP_000124150.1	terminase	-
BJJ90_RS29435	128603..128716	+	114	WP_075362742.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / aac(3)-IVa / fosA3 / blaCTX-M-14 / mph(A)	-	1..369298	369298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T67844 WP_001216034.1 NZ_CP017632:123441-123821 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T67844 NZ_CP017632:123441-123821 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCTTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT67844 WP_001190712.1 NZ_CP017632:123220-123441 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT67844 NZ_CP017632:123220-123441 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6