Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4472487..4473063 Replicon chromosome
Accession NZ_CP017631
Organism Escherichia coli strain SLK172

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag BJJ90_RS24260 Protein ID WP_001297643.1
Coordinates 4472776..4473063 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag BJJ90_RS24255 Protein ID WP_000063156.1
Coordinates 4472487..4472789 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJJ90_RS24240 4469083..4471233 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
BJJ90_RS24245 4471283..4471486 + 204 WP_000467859.1 YbdD/YjiX family protein -
BJJ90_RS24250 4471497..4472453 + 957 WP_075362688.1 GTPase -
BJJ90_RS24255 4472487..4472789 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
BJJ90_RS24260 4472776..4473063 - 288 WP_001297643.1 BrnT family toxin Toxin
BJJ90_RS24265 4473262..4473959 - 698 Protein_4306 IS1 family transposase -
BJJ90_RS32270 4474016..4474111 + 96 Protein_4307 dienelactone hydrolase family protein -
BJJ90_RS24275 4474257..4475489 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
BJJ90_RS24280 4475530..4476810 + 1281 WP_069722975.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T67839 WP_001297643.1 NZ_CP017631:c4473063-4472776 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T67839 NZ_CP017631:c4473063-4472776 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT67839 WP_000063156.1 NZ_CP017631:c4472789-4472487 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT67839 NZ_CP017631:c4472789-4472487 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References