Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2475174..2475812 Replicon chromosome
Accession NZ_CP017631
Organism Escherichia coli strain SLK172

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BJJ90_RS13345 Protein ID WP_000813794.1
Coordinates 2475174..2475350 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BJJ90_RS13350 Protein ID WP_001270286.1
Coordinates 2475396..2475812 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJJ90_RS13325 2470793..2472007 - 1215 WP_001326689.1 BenE family transporter YdcO -
BJJ90_RS13330 2472060..2472596 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
BJJ90_RS13335 2472669..2474630 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BJJ90_RS13340 2474722..2474952 - 231 WP_000494244.1 YncJ family protein -
BJJ90_RS13345 2475174..2475350 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BJJ90_RS13350 2475396..2475812 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BJJ90_RS13355 2475891..2477300 + 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
BJJ90_RS13360 2477542..2478687 + 1146 WP_000047429.1 ABC transporter substrate-binding protein -
BJJ90_RS13365 2478705..2479718 + 1014 WP_000220393.1 ABC transporter ATP-binding protein -
BJJ90_RS13370 2479719..2480660 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - vgrG/tssI / vgrG/tssI / rhs/PAAR / espR1 / espR1 / sodB 2331608..2698747 367139


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T67824 WP_000813794.1 NZ_CP017631:2475174-2475350 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T67824 NZ_CP017631:2475174-2475350 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT67824 WP_001270286.1 NZ_CP017631:2475396-2475812 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT67824 NZ_CP017631:2475396-2475812 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References