Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2368646..2369172 Replicon chromosome
Accession NZ_CP017631
Organism Escherichia coli strain SLK172

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BJJ90_RS12810 Protein ID WP_000323025.1
Coordinates 2368885..2369172 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BJJ90_RS12805 Protein ID WP_000534858.1
Coordinates 2368646..2368885 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJJ90_RS12745 2363915..2365189 + 1275 WP_075362599.1 ISNCY family transposase -
BJJ90_RS12770 2367005..2367256 - 252 WP_075362600.1 FlxA-like family protein -
BJJ90_RS12775 2367275..2367972 - 698 Protein_2265 IS1-like element IS1A family transposase -
BJJ90_RS12790 2368027..2368113 - 87 Protein_2266 protein FlxA -
BJJ90_RS12800 2368316..2368621 - 306 WP_071600497.1 hypothetical protein -
BJJ90_RS12805 2368646..2368885 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BJJ90_RS12810 2368885..2369172 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BJJ90_RS12815 2369244..2369399 + 156 WP_000813269.1 type I toxin-antitoxin system toxin HokD -
BJJ90_RS12820 2369868..2370467 + 600 WP_025670565.1 DUF1367 family protein -
BJJ90_RS12830 2370589..2371611 + 1023 WP_000255944.1 IS21-like element IS100 family transposase -
BJJ90_RS12835 2371608..2372405 + 798 WP_075362601.1 IS21-like element IS100kyp family helper ATPase IstB -
BJJ90_RS12855 2373772..2374029 + 258 WP_069723324.1 DUF1364 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - vgrG/tssI / vgrG/tssI / rhs/PAAR / espR1 / espR1 / sodB 2331608..2698747 367139
- inside IScluster/Tn - - 2360310..2373621 13311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T67823 WP_000323025.1 NZ_CP017631:2368885-2369172 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T67823 NZ_CP017631:2368885-2369172 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT67823 WP_000534858.1 NZ_CP017631:2368646-2368885 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT67823 NZ_CP017631:2368646-2368885 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References