Detailed information of TA system TA067809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1331723..1332258
• Replicon: chromosome
• Accession: NZ_CP017622
• Organism: Ketogulonicigenium vulgare strain SPU B805

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KVC_RS06690
• Protein ID: WP_013384044.1
• Coordinates: 1332010..1332258 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: F9Y4N2
• Locus tag: KVC_RS06685
• Protein ID: WP_013384043.1
• Coordinates: 1331723..1332013 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVC_RS06645	1326859..1327263	+	405	WP_013384036.1	hypothetical protein	-
KVC_RS06650	1327260..1328414	+	1155	WP_013384037.1	hypothetical protein	-
KVC_RS06655	1328414..1328677	+	264	WP_013384038.1	hypothetical protein	-
KVC_RS06660	1328790..1329419	+	630	WP_044008023.1	TetR/AcrR family transcriptional regulator	-
KVC_RS06665	1329419..1329754	+	336	WP_014537641.1	DoxX family protein	-
KVC_RS06670	1329902..1330165	+	264	WP_013384040.1	hypothetical protein	-
KVC_RS06675	1330165..1331469	+	1305	WP_065739317.1	hypothetical protein	-
KVC_RS06680	1331484..1331711	+	228	WP_013384042.1	hypothetical protein	-
KVC_RS06685	1331723..1332013	-	291	WP_013384043.1	BrnA antitoxin family protein	Antitoxin
KVC_RS06690	1332010..1332258	-	249	WP_013384044.1	BrnT family toxin	Toxin
KVC_RS06695	1332355..1332897	+	543	WP_013384045.1	lysozyme	-
KVC_RS06700	1332901..1333122	+	222	WP_013384046.1	hypothetical protein	-
KVC_RS15565	1333122..1333337	+	216	WP_162467560.1	hypothetical protein	-
KVC_RS06710	1333528..1333986	-	459	WP_014537644.1	sigma-70 family RNA polymerase sigma factor	-
KVC_RS06715	1334288..1335214	-	927	WP_065739318.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1292777..1333473	40696

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9028.54 Da
• Isoelectric Point: 7.3199

>T67809 WP_013384044.1 NZ_CP017622:c1332258-1332010 [Ketogulonicigenium vulgare]
MMMIVWDEPKRESNLEKHGLDFADLSEEFFLSSVVVPAKNGRHMAIGKLADGTIAVVFATLGTQGLSVISMRHASKKERA
IL

Nucleotide

• Length: 249 bp

>T67809 NZ_CP017622:c1332258-1332010 [Ketogulonicigenium vulgare]
ATGATGATGATTGTCTGGGATGAACCGAAACGCGAAAGCAACCTCGAGAAACACGGGTTAGATTTCGCAGATTTGTCCGA
GGAATTCTTTCTGTCCTCGGTCGTTGTCCCGGCAAAGAACGGGCGTCACATGGCGATAGGGAAGCTTGCCGACGGAACGA
TTGCAGTGGTCTTTGCCACACTTGGAACGCAAGGCCTGTCGGTGATTTCGATGCGCCACGCCAGCAAAAAGGAAAGGGCG
ATCCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10567.07 Da
• Isoelectric Point: 10.4185

>AT67809 WP_013384043.1 NZ_CP017622:c1332013-1331723 [Ketogulonicigenium vulgare]
MKANKLTEKEIQRRIASDPDAPEATDDQLAKAKPFAEAMPQLAENLRRNLGGRPKSDNPKVAVSLRLDQDVIEKFKAAGP
GWQSRMNKALREAAGV

Nucleotide

• Length: 291 bp

>AT67809 NZ_CP017622:c1332013-1331723 [Ketogulonicigenium vulgare]
ATGAAAGCCAACAAGCTAACCGAGAAAGAAATCCAACGCCGGATCGCAAGCGACCCGGACGCACCCGAGGCAACAGATGA
CCAGCTGGCGAAGGCGAAGCCCTTCGCCGAGGCCATGCCCCAACTGGCAGAGAACCTGCGCCGCAACCTTGGCGGCCGCC
CGAAATCCGACAACCCCAAAGTCGCGGTTTCGCTGCGCCTCGATCAAGACGTGATTGAGAAGTTCAAGGCCGCTGGCCCC
GGCTGGCAGTCGCGCATGAACAAAGCCCTGCGCGAAGCTGCGGGGGTATGA

Structures

Antitoxin

• AlphaFold DB: F9Y4N2