Detailed information of TA system TA067808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26460..26985
• Replicon: plasmid unnamed1
• Accession: NZ_CP017620
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain 22792

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: BKC12_RS00165
• Protein ID: WP_001159863.1
• Coordinates: 26680..26985 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: BKC12_RS00160
• Protein ID: WP_000813641.1
• Coordinates: 26460..26678 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKC12_RS00130	21747..22128	+	382	Protein_23	YgiW/YdeI family stress tolerance OB fold protein	-
BKC12_RS25450	22405..22741	+	337	Protein_24	site-specific integrase	-
BKC12_RS00140	23000..23989	-	990	Protein_25	RepB family plasmid replication initiator protein	-
BKC12_RS25020	24483..24779	-	297	WP_001687482.1	hypothetical protein	-
BKC12_RS00155	25263..25784	-	522	WP_010999942.1	hypothetical protein	-
BKC12_RS00160	26460..26678	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BKC12_RS00165	26680..26985	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
BKC12_RS00170	26987..27277	+	291	WP_001266176.1	hypothetical protein	-
BKC12_RS00175	27274..27795	+	522	WP_000198608.1	hypothetical protein	-
BKC12_RS00180	27830..28612	+	783	WP_000082169.1	site-specific integrase	-
BKC12_RS00185	28621..29294	+	674	Protein_33	EAL domain-containing protein	-
BKC12_RS00190	29298..29807	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
BKC12_RS00195	29801..30286	+	486	WP_000905606.1	membrane protein	-
BKC12_RS25455	30563..30850	-	288	WP_071530243.1	hypothetical protein	-
BKC12_RS00200	31006..31566	+	561	WP_071953347.1	inverse autotransporter beta domain-containing protein	-
BKC12_RS00205	31633..31983	-	351	WP_001541541.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93595	93595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T67808 WP_001159863.1 NZ_CP017620:26680-26985 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T67808 NZ_CP017620:26680-26985 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT67808 WP_000813641.1 NZ_CP017620:26460-26678 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT67808 NZ_CP017620:26460-26678 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6