Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 452243..452870 Replicon chromosome
Accession NZ_CP017603
Organism Clostridium formicaceticum strain ATCC 27076

Toxin (Protein)


Gene name pemK Uniprot ID A0A1D9FHI3
Locus tag BJL90_RS02010 Protein ID WP_070963824.1
Coordinates 452243..452593 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1D9FHE6
Locus tag BJL90_RS02015 Protein ID WP_044824195.1
Coordinates 452595..452870 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJL90_RS01995 447799..449979 - 2181 WP_070963819.1 hypothetical protein -
BJL90_RS02000 450222..451286 - 1065 WP_169824185.1 acyltransferase -
BJL90_RS02005 451470..451961 - 492 WP_070963822.1 hypothetical protein -
BJL90_RS02010 452243..452593 - 351 WP_070963824.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BJL90_RS02015 452595..452870 - 276 WP_044824195.1 ribbon-helix-helix protein, CopG family Antitoxin
BJL90_RS02020 453023..454195 - 1173 WP_070963826.1 alanine racemase -
BJL90_RS02025 454370..454999 - 630 WP_070963827.1 outer membrane lipoprotein carrier protein LolA -
BJL90_RS02030 455130..455498 - 369 WP_070963829.1 holo-ACP synthase -
BJL90_RS02035 455518..456141 - 624 WP_070963831.1 hypothetical protein -
BJL90_RS02040 456420..457409 + 990 WP_070963833.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12806.86 Da        Isoelectric Point: 5.1570

>T67807 WP_070963824.1 NZ_CP017603:c452593-452243 [Clostridium formicaceticum]
MVVKRGDIYYADLSPVIGSEQGGTRPVLVVQNDIGNRYSPTVIIAAITSQINKAKLPTHVEIAASEYGLTKDSVILLEQI
RTIDKRRLEEKIGHLDDEMMIKVSEALLISLGLVDF

Download         Length: 351 bp

>T67807 NZ_CP017603:c452593-452243 [Clostridium formicaceticum]
TTGGTTGTTAAAAGAGGCGACATATATTATGCAGACTTAAGTCCTGTTATAGGATCTGAGCAGGGGGGAACCAGACCCGT
TCTTGTTGTTCAAAATGATATAGGCAATCGATATAGTCCTACAGTTATTATTGCAGCTATTACTTCTCAAATCAATAAAG
CCAAGCTTCCTACCCATGTAGAAATTGCAGCCTCAGAATATGGATTAACAAAAGATTCGGTTATACTGCTAGAACAAATT
AGAACAATTGATAAAAGAAGATTAGAAGAAAAGATAGGTCATTTAGATGATGAAATGATGATAAAGGTAAGTGAGGCACT
TTTGATAAGCTTAGGGTTAGTAGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10539.18 Da        Isoelectric Point: 4.5214

>AT67807 WP_044824195.1 NZ_CP017603:c452870-452595 [Clostridium formicaceticum]
MADSKRIMISLPESLLKEVDYIVSMEETNRSEFVREAMKLYIREKNKVKLREKMKKGYQEMASINLTLAEVGLSLDVSSL
EDYEAEIAECE

Download         Length: 276 bp

>AT67807 NZ_CP017603:c452870-452595 [Clostridium formicaceticum]
ATGGCTGATTCTAAAAGGATAATGATTAGTCTTCCTGAAAGTCTTCTTAAAGAAGTAGATTATATAGTTTCTATGGAGGA
AACCAATAGAAGTGAATTTGTCAGGGAAGCCATGAAACTATATATAAGGGAAAAAAACAAAGTAAAACTAAGAGAAAAAA
TGAAAAAGGGCTATCAAGAAATGGCTTCAATCAACTTAACATTGGCAGAGGTAGGGTTAAGTCTAGATGTGTCCTCCCTA
GAAGATTATGAGGCAGAAATAGCGGAGTGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D9FHI3

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9FHE6

References