Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2867630..2868261 Replicon chromosome
Accession NZ_CP017598
Organism Mycobacterium tuberculosis strain Beijing-like/1104

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BJM04_RS13970 Protein ID WP_003413174.1
Coordinates 2867884..2868261 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BJM04_RS13965 Protein ID WP_003413167.1
Coordinates 2867630..2867887 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM04_RS13930 2863544..2863858 + 315 WP_009937839.1 hypothetical protein -
BJM04_RS13935 2864154..2865365 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM04_RS13945 2865492..2866151 + 660 WP_003902296.1 hypothetical protein -
BJM04_RS13950 2866148..2866811 + 664 Protein_2629 hypothetical protein -
BJM04_RS21500 2866808..2867085 + 278 Protein_2630 type II toxin-antitoxin system VapB family antitoxin -
BJM04_RS13960 2867178..2867591 + 414 WP_003413164.1 PIN domain nuclease -
BJM04_RS13965 2867630..2867887 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BJM04_RS13970 2867884..2868261 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM04_RS13975 2868277..2868651 - 375 WP_003413177.1 hypothetical protein -
BJM04_RS13980 2868751..2869146 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BJM04_RS13985 2869143..2869388 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BJM04_RS13995 2869799..2870218 - 420 WP_003413190.1 A24 family peptidase -
BJM04_RS14000 2870230..2871039 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM04_RS14005 2871036..2872289 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM04_RS14010 2872282..2872794 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T67782 WP_003413174.1 NZ_CP017598:2867884-2868261 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T67782 NZ_CP017598:2867884-2868261 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT67782 WP_003413167.1 NZ_CP017598:2867630-2867887 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT67782 NZ_CP017598:2867630-2867887 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References