Detailed information of TA system TA067768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2186128..2186831
• Replicon: chromosome
• Accession: NZ_CP017598
• Organism: Mycobacterium tuberculosis strain Beijing-like/1104

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BJM04_RS10485
• Protein ID: WP_003409778.1
• Coordinates: 2186128..2186457 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BJM04_RS10490
• Protein ID: WP_003409780.1
• Coordinates: 2186454..2186831 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM04_RS10465	2182511..2183581	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BJM04_RS10470	2183578..2184093	+	516	WP_003409718.1	flavin reductase family protein	-
BJM04_RS10475	2184090..2185151	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BJM04_RS10480	2185148..2185918	+	771	WP_003409775.1	SDR family oxidoreductase	-
BJM04_RS10485	2186128..2186457	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJM04_RS10490	2186454..2186831	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BJM04_RS10495	2186828..2187418	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BJM04_RS10500	2187473..2188837	+	1365	WP_003903691.1	HNH endonuclease	-
BJM04_RS10505	2188992..2189444	-	453	WP_003899095.1	lipoprotein	-
BJM04_RS10510	2189508..2189909	+	402	WP_003409869.1	hypothetical protein	-
BJM04_RS10515	2189902..2190084	-	183	WP_003409870.1	hypothetical protein	-
BJM04_RS10520	2190198..2190548	-	351	WP_003409871.1	hypothetical protein	-
BJM04_RS10525	2190559..2191461	-	903	WP_003899097.1	hypothetical protein	-
BJM04_RS10530	2191482..2191673	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T67768 WP_003409778.1 NZ_CP017598:c2186457-2186128 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T67768 NZ_CP017598:c2186457-2186128 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT67768 WP_003409780.1 NZ_CP017598:c2186831-2186454 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT67768 NZ_CP017598:c2186831-2186454 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8