Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3155360..3155964 Replicon chromosome
Accession NZ_CP017597
Organism Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag BJM03_RS15565 Protein ID WP_003414492.1
Coordinates 3155360..3155752 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag BJM03_RS15570 Protein ID WP_003414495.1
Coordinates 3155749..3155964 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM03_RS15535 3150510..3151298 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
BJM03_RS15540 3151632..3152177 - 546 WP_014584866.1 DUF1802 family protein -
BJM03_RS15545 3152449..3153333 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
BJM03_RS15550 3153336..3154223 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
BJM03_RS15555 3154528..3155073 - 546 WP_003910939.1 DUF1802 family protein -
BJM03_RS15560 3155070..3155339 - 270 WP_003414489.1 DUF2277 family protein -
BJM03_RS15565 3155360..3155752 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM03_RS15570 3155749..3155964 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BJM03_RS15575 3156011..3156760 + 750 WP_003902358.1 enoyl-CoA hydratase -
BJM03_RS15580 3156839..3157921 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
BJM03_RS15585 3157914..3159224 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
BJM03_RS15590 3159227..3160054 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
BJM03_RS15595 3160051..3160962 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T67730 WP_003414492.1 NZ_CP017597:c3155752-3155360 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T67730 NZ_CP017597:c3155752-3155360 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT67730 WP_003414495.1 NZ_CP017597:c3155964-3155749 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT67730 NZ_CP017597:c3155964-3155749 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References