Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2894894..2895525 Replicon chromosome
Accession NZ_CP017597
Organism Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BJM03_RS14085 Protein ID WP_003413174.1
Coordinates 2895148..2895525 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BJM03_RS14080 Protein ID WP_003413167.1
Coordinates 2894894..2895151 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM03_RS14045 2890808..2891122 + 315 WP_009937839.1 hypothetical protein -
BJM03_RS14050 2891418..2892629 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM03_RS14060 2892756..2893415 + 660 WP_003902296.1 hypothetical protein -
BJM03_RS14065 2893412..2894075 + 664 Protein_2655 hypothetical protein -
BJM03_RS21730 2894072..2894349 + 278 Protein_2656 type II toxin-antitoxin system VapB family antitoxin -
BJM03_RS14075 2894442..2894855 + 414 WP_003413164.1 PIN domain nuclease -
BJM03_RS14080 2894894..2895151 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BJM03_RS14085 2895148..2895525 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM03_RS14090 2895541..2895915 - 375 WP_003413177.1 hypothetical protein -
BJM03_RS14095 2896015..2896410 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BJM03_RS14100 2896407..2896652 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BJM03_RS14110 2897063..2897482 - 420 WP_003413190.1 A24 family peptidase -
BJM03_RS14115 2897494..2898303 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM03_RS14120 2898300..2899553 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM03_RS14125 2899546..2900058 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T67722 WP_003413174.1 NZ_CP017597:2895148-2895525 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T67722 NZ_CP017597:2895148-2895525 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT67722 WP_003413167.1 NZ_CP017597:2894894-2895151 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT67722 NZ_CP017597:2894894-2895151 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References